真菌出芽短梗霉中一个新的酚苷类化合物

A new phenolic glycoside from the fungus Aureobasidium pullulans

真菌出芽短梗霉Aureobasidium pullulans乙酸乙酯粗提物对多种植物病原菌具有抑菌活性,结果表明对人参锈腐病病原菌Cylindrocarpon destructans具有较好的抑菌活性。因此为探究出芽短梗霉A.pullulans固体发酵物乙酸乙酯部位的化学成分,对其发酵物乙酸乙酯部位采用正相柱,反相柱,以及半制备液相色谱分离纯化,并根据核磁共振波谱数据鉴定单体化合物。分离得到4个单体化合物,经鉴定为3,4-二甲氧基-5-甲基苯-O-α-D-吡喃葡萄糖苷(1)、5′-hydroxygriseofulvin(2)、7-dechlorogriseofulvin(3)、6′-hydroxygriseofulvin(4)。以上4种化合物均为首次从该真菌发酵产物中分离得到,其中化合物1是新化合物。

Crude samples from Aureobasidium pullulans fermentation products extracted using ethyl acetate exhibited inhibitory activity against a variety of plant pathogens,and the results showed good inhibitory activity against Cylindrocarpon destructans.To further explore the chemical constituents of A.pullulans fermentation products,ethyl acetate extract from the solid culture were separated and purified using normal-phase silica columns,reversed-phase silica columns and semi-preparative liquid chromatography,the monomer compounds were then determined mainly through analysis of NMR spectroscopic data.Four compounds were found and identified as 3,4-dimethoxy-5-methylbenzene-O-α-D-glucopyranoside(1),5′-hydroxygriseofulvin(2),7-dechlorogriseofulvin(3)and 6′-hydroxygriseofulvin(4).The above four compounds were isolated from the fermentation products of this fungus for the first time,and compound 1 was a new compound.