摘要
采用高效液相色谱法建立水蛭药材特征图谱并同时测定5种有效成分的含量,为不同基原水蛭及其混淆品的质量评价提供依据。采用中药色谱指纹图谱相似度评价系统(2012版本)软件对特征图谱结果进行分析,结果显示,27批蚂蟥HPLC特征图谱具有8个共有峰,并通过对照品对比确认其中5个成分,分别为:尿嘧啶、次黄嘌呤、黄嘌呤、水蛭胺C羧基衍生物、水蛭胺B;采用SIMCA-P 14.0软件进行聚类及偏最小二乘法分析,均可将不同基原水蛭及其混淆品归为不同的四类;另外,含量测定结果显示,不同基原水蛭及其混淆品之间的含量存在一定差异,尿嘧啶、次黄嘌呤的含量均值依次为:蚂蟥>水蛭>柳叶蚂蟥>菲牛蛭;黄嘌呤的含量均值依次为:蚂蟥>水蛭>菲牛蛭>柳叶蚂蟥;水蛭胺C羧基衍生物的含量均值依次为:柳叶蚂蟥>蚂蟥>水蛭>菲牛蛭;水蛭胺B的含量均值依次为:柳叶蚂蟥>蚂蟥>水蛭>菲牛蛭。本研究所建立的方法可作为不同基原水蛭及其混淆品的质量评价方法,为水蛭药材的质量评价提供参考依据。
HPLC method was used to establish the characteristic chromatogram of Hirudo and the contents of 5 components were determined,which provides a basis for quality evaluation of different origins Hirudo and its adulterants.The Chinese medicine chromatographic fingerprint similarity evaluation system(2012 version)was used to analyze the results of the characteristic chromatogram.The results showed that there were eight common peaks in 27 batches of Whitmania pigra Whitman and five peaks were identified by comparison with reference substances,respectively,which were uracil,hypoxanthine,xanthine,hirudine C carboxyl derivatives and hirudine B.The SIMCA-P 14.0 software were used for cluster analysis and partial least squares discriminant analysis(PLS-DA)that both of them can distinguish Hirudo of different origins and its adulterants into four different classes.In addition,the content determination results showed that there were some differences in the content of Hirudo with different origins and its adulterants.The average content of uracil and hypoxanthine was in the order of Whitmania pigra Whitman>Hirudo nipponica Whitman>Whitmania acranulata Whitman>Poecilobdella manillensis Lesson;the average content of xanthine was in the order of Whitmania pigra Whitman>Hirudo nipponica Whitman>Poecilobdella manillensis Lesson>Whitmania acranulata Whitman;the average content of hirudine C carboxyl derivatives was in the order of Whitmania acranulata Whitman>Whitmania pigra Whitman>Hirudo nipponica Whitman>Poecilobdella manillensis Lesson;the average content of hirudine B was in order of Whitmania acranulata Whitman>Whitmania pigra Whitman>Hirudo nipponica Whitman>Poecilobdella manillensis Lesson.The method established in this study can be used as a quality evaluation method for Hirudo with different origins and its adulterants,and provide a reference for the quality evaluation of Whitmania pigra Whitman.
作者
李国卫
邱韵静
童培珍
胡绮萍
邓立萍
杨丽
孙冬梅
LI Guo-wei;QIU Yun-jing;TONG Pei-zhen;HU Qi-ping;DENG Li-ping;YANG Li;SUN Dong-mei(Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第6期997-1006,共10页
Natural Product Research and Development
基金
广东省基础与应用基础研究基金(2020B1515120033)。
关键词
水蛭
基原
聚类分析
含量测定
特征图谱
Hirudo
origin
cluster analysis
content determination
HPLC characteristic chromatogram