溪黄草药材4种基原植物的UPLC特征图谱鉴别研究

Identification of UPLC characteristics spectrum of four original plants of Rabdosiae Herba

本研究采用UPLC法建立适用于鉴别溪黄草药材4种基原植物溪黄草Rabdosia serra(Maxim.)H.Hara、线纹香茶菜Rabdosia lophanthoides(Buch.-Ham.ex D.Don)H.Hara、纤花香茶菜Rabdosia lophanthoides var.graciliflora(Benth.)H.Hara和长叶香茶菜Rabdosia stracheyi(Benth.ex Hook.f.)Hara的特征图谱,并结合特征图谱的相似度评价、聚类分析(cluster analysis,CA)、主成分分析(principal component analysis,PCA)以及正交偏最小二乘法判别分析(orthogonal partial least squares-discriminate analysis,OPLS-DA)对4种基原植物的共有成分与差异性成分进行研究。从溪黄草、线纹香茶菜、纤花香茶菜和长叶香茶菜的特征图谱中分别标定了19、24、26、21个特征峰。除溪黄草外,其他3种基原植物各自样品的相似度较高;但是不同基原植物之间的相似度具有一定的差异性。通过CA、PCA可将溪黄草与其他3种基原植物明显区分,通过OPLS-DA可将线纹香茶菜、纤花香茶菜和长叶香茶菜区分。该研究建立的UPLC特征图谱结合化学计量学方法较全面地反映了溪黄草药材4种基原植物的化学成分,方法简便快速、专属性强,可为溪黄草药材的基原鉴别和质量分析与评价提供参考。

The purpose of this paper is to establish the UPLC characteristics spectrum method for the identification of four original plants of Rabdosiae Herba,Rabdosia serra(Maxim.)H.Hara,Rabdosia lophanthoides(Buch.-Ham.ex D.Don)H.Hara,Rabdosia lophanthoides var.graciliflora(Benth.)H.Hara and Rabdosia stracheyi(Benth.ex Hook.f.)Hara.The similarity evaluation of characteristic chromatograms,cluster analysis(CA),principal component analysis(PCA)and orthogonal partial least square discriminant analysis(OPLS-DA)were combined,which was used to study the common components and differential components of four original plants of Rabdosiae Herba.19,24,26 and 21 characteristic peaks were determined from the characteristic chromatograms of R.serra,R.lophanthoides,R.lophanthoides var.graciliflora and R.stracheyi.In addition to R.serra,the similarity of the samples of the other three original plants was high,but there were some differences in similarity between different original plants.R.serra could be clearly distinguished from the other three original plants by CA and PCA and OPLS-DA could be used to distinguish R.lophanthoides,R.lophanthoides var.graciliflora and R.stracheyi.The UPLC characteristics spectrum established in this study combined with chemometrics comprehensively reflected the chemical composition of four original plants of Rabdosiae Herba,and the method is simple,rapid and specific,which can provide a reference for the identification,quality analysis and evaluation of original plants of Rabdosiae Herba.