肺癌组织miR-29a-3p、TRIB2水平及其与患者病理特征、预后的关系

Levels of miR-29a-3p and TRIB2 in lung cancer tissues and their relationship with pathological characteristics and prognosis of patients

目的:探讨微小RNA-29a-3p(miR-29a-3p)、tribbles同源蛋白2(TRIB2)在肺癌组织中的表达情况,分析其与患者临床病理特征和预后的关系。方法:选取2017年7月至2018年10月在本院进行手术治疗的80例肺癌患者的癌组织及其癌旁组织(距癌组织>5 cm)样本进行研究。采用实时荧光定量PCR技术检测肺癌组织及癌旁组织中miR-29a-3p、TRIB2 mRNA表达情况;采用Pearson相关性分析miR-29a-3p和TRIB2之间的相关性;收集肺癌患者临床病理特征,分析miR-29a-3p和TRIB2表达水平与临床病理特征的关系,采用Kaplan-Meier法分析miR-29a-3p和TRIB2表达水平与肺癌患者3年生存率的关系,采用多因素Cox回归分析肺癌患者预后影响因素。结果:肺癌组织miR-29a-3p表达低于癌旁组织,TRIB2 mRNA表达高于癌旁组织,差异具有统计学意义(P<0.05);肺癌组织miR-29a-3p和TRIB2 mRNA表达与肺癌患者TNM分期、分化程度和淋巴结转移有关(P<0.05);肺癌组织miR-29a-3p与TRIB2 mRNA表达呈负相关(r=-0.566,P<0.05);miR-29a-3p高表达组患者3年生存率(88.00%)高于低表达组患者(33.33%),TRIB2低表达组患者3年生存率(87.76%)高于高表达组患者(35.48%),差异具有统计学意义(P<0.05);肺癌患者TNM分期、分化程度、淋巴结转移、miR-29a-3p和TRIB2 mRNA水平均为患者预后影响因素(P<0.05)。结论:肺癌组织中miR-29a-3p表达下调,TRIB2 mRNA表达上调,与肺癌患者TNM分期、分化程度和淋巴结转移有关,二者表达水平可能与患者预后情况密切相关。

Objective:To investigate the expression of microRNA-29a-3p(miR-29a-3p)and tribbles homologue 2(TRIB2)in lung cancer tissues,and to analyze their relationship with clinicopathological characteristics and prognosis of patients.Methods:The cancer tissues and their adjacent tissues(>5 cm away from the cancer tissues)samples from 80 patients with lung cancer who underwent surgery in our hospital from July 2017 to October 2018 were gathered for research.Real-time fluorescence quantitative PCR technology was applied to detect the expression of miR-29a-3p and TRIB2 mRNA in lung cancer tissues and adjacent tissues;Pearson correlation was applied to analyze the correlation between miR-29a-3p and TRIB2;clinicopathological characteristics of lung cancer patients were collected,the relationship between miR-29a-3p,TRIB2 expression levels and clinicopathological features was analyzed,Kaplan-Meier method was applied to analyze the relationship between the expression levels of miR-29a-3p,TRIB2 and the 3-year survival rate of lung cancer patients,multivariate Cox regression was applied to analyze prognostic factors in lung cancer patients.Results:The expression of miR-29a-3p in lung cancer tissues was lower than that in adjacent tissues,and the expression of TRIB2 mRNA was higher than that in adjacent tissues(P<0.05);the expression of miR-29a-3p and TRIB2 mRNA in lung cancer tissues was related to TNM stage,degree of differentiation and lymph node metastasis in lung cancer patients(P<0.05);there was a negative correlation between miR-29a-3p and TRIB2 mRNA expression in lung cancer tissues(r=-0.566,P<0.05);the 3-year survival rate of patients in high miR-29a-3p expression group(88.00%)was higher than that of patients in low expression group(33.33%),and the 3-year survival rate of patients in low TRIB2 expression group(87.76%)was higher than that of patients in high expression group(35.48%)(P<0.05);TNM stage,degree of differentiation,lymph node metastasis,miR-29a-3p and TRIB2 mRNA levels in lung cancer patients were all prognostic factors(P<0.05).Conclusion:The expression of miR-29a-3p is down-regulated in lung cancer tissues,and the expression of TRIB2 mRNA is up-regulated.They are related to TNM stage,degree of differentiation and lymph node metastasis of lung cancer patients,and their expression levels may be closely related to the prognosis of patients.