急性肾损伤患者血清KLF8和CXCL8水平与肠道菌群的关系研究

Study on the relationship between serum KLF8 and CXCL8 levels and intestinal flora in patients with acute renal injury

目的探讨急性肾损伤患者血清Kruppel样转录因子8(KLF8)和CXC趋化因子配体8(CXCL8)水平与肠道菌群的关系。方法选取2020年2月-2022年2月本院收治的80例急性肾损伤患者作为实验组,同期健康体检志愿者80例作为对照组。采用荧光定量RT-PCR检测KLF8和CXCL8mRNA表达,取患者粪便检测肠道菌群的数量,比较不同组别血清KLF8和CXCL8表达的差异和粪便肠道菌群的数量差异;对可能影响急性肾损伤发病的影响因素进行Logistic回归分析;采用Spearman相关性分析KLF8和CXCL8表达与肠道菌群的相关性。结果和对照组相比,实验组患者血清KLF8和CXCL8水平明显升高,粪便肠道菌群双歧杆菌、拟杆菌明显降低,大肠埃希菌、链球菌明显升高(P<0.05);Ⅰ期组、Ⅱ期组、Ⅲ期组血清KLF8、CXCL8mRNA水平依次显著升高(P<0.05),粪便双歧杆菌、拟杆菌水平依次显著降低,大肠埃希菌、链球菌水平依次显著升高(P<0.05);Logistic回归分析发现,双歧杆菌、拟杆菌是影响急性肾损伤发病的保护因素(P<0.05);KLF8和CXCL8的表达与双歧杆菌、拟杆菌呈负相关,与大肠埃希菌、链球菌呈正相关。结论急性肾损伤患者血清KLF8和CXCL8水平显著升高,其与肠道菌群异常有关。

Objective To investigate the relationship between the levels of serum Kruppel-like transcription factor 8(KLF8)and CXC chemokine ligand 8(CXCL8)and intestinal flora in patients with acute renal injury.Methods Eighty patients with acute renal injury admitted to our hospital from February 2020 to February 2022 were collected as the experimental group,and 80 healthy volunteers in the same period were collected as the control group.The expression of KLF8 and CXCL8 mRNA was detected by fluorescence quantitative RT-PCR,the stool of the patient was taken to detect the number of intestinal flora,the differences in the expression of KLF8 and CXCL8 in serum and the number of intestinal microflora in feces among different groups were compared;Logistic regression analysis was performed on the factors that might affect the incidence of acute renal injury;Spearman correlation was applied to analyze the correlation between KLF8 and CXCL8 expression and intestinal flora.Results Compared with the control group,the levels of serum KLF8 and CXCL8 in the experimental group were obviously higher,the fecal intestinal microflora bifidobacteria and bacteroides were obviously lower,and the levels of Escherichia coli and streptococcus were obviously higher(P<0.05);The mRNA levels of serum KLF8 and CXCL8 in stageⅠ,ⅡandⅢgroups were significantly increased(P<0.05),the levels of fecal bifidobacterium and Bacteroides were significantly decreased,and the levels of E.coli and Streptococcus were significantly increased(P<0.05);Logistic regression analysis showed that bifidobacterium and bacteroides were protective factors affecting the pathogenesis of acute renal injury(P<0.05);the expression of KLF8 and CXCL8 was negatively correlated with Bifidobacteria and Bacteroides,and positively correlated with Escherichia coli and Streptococcus.Conclusion The levels of serum KLF8 and CXCL8 in patients with acute renal injury are obviously increased,which is related to the abnormality of intestinal flora.