彭布洛克威尔士柯基犬19个STR基因座的多态性研究

Genetic Polymorphisms of 19 STR Loci in Pembroke Welsh Corgi

目的对彭布洛克威尔士柯基犬(以下简称柯基犬)的19个STR基因座进行遗传多态性研究。方法选用犬荧光标记19个STR基因座复合扩增试剂盒进行PCR扩增,并进行统计学分析。结果对212只纯种柯基犬的19个基因座的多态性进行统计学分析,经Hardy-Weinberg平衡检验,除PEZ20基因座外,P值均大于0.05,18个基因座的累积非父排除率(CPE)为0.9998289783352165,累积个体识别率(TDP)为0.999999999999998,平均杂合度和平均多态信息含量分别为0.631和0.604。结论19个STR基因座中的18个(PEZ20基因座除外)联合应用可以用于柯基犬的个体识别和亲权鉴定。

Objective To explore the genetic polymorphisms of 19 STR loci of the Pembroke Welsh Corgi(Hereinafter referred to as corgi).Method The PCR amplification was performed using the 19 STR fluorescent multiple amplification kit for dog.The amplified products were detected and statistically analyzed.Result To analysis the genetic polymorphisms of 19 loci of 212 purebred corgis,using Hardy-Weinberg equilibrium testing,all loci had P values greater than 0.05 except PEZ20,the Cumulative PE(CPE)is 0.9998289783352165 and the Total Power of Discrimination(TDP)is 0.999999999999998 by 18 STR loci,the average Heterozygosity is 0.631 and the average Polymorphism Information Content is 0.604.Conclusion 18 of the 19 STR loci(except PEZ20)can be applied to individual discrimination and paternity test of corgi.