舒泰50与速眠新Ⅱ混合配比对SPF小鼠的麻醉效果

Effect of Mixed Ratio of Zoletil 50 and Sumianxin II on Anesthesia in SPF Mice

目的探索新型非管制麻醉药舒泰50与速眠新Ⅱ混合配比对SPF小鼠的麻醉效果,希望能找到一种对各品系小鼠都能达到理想麻醉效果的方法。方法参照药物说明书,以犬猫兔用量为参考,分别单用速眠新Ⅱ(30倍稀释液),单用舒泰50(10倍稀释液),速眠新Ⅱ与舒泰50各稀释10倍按1∶1混合配比,速眠新Ⅱ(20倍稀释液)与舒泰50(5倍稀释液)1∶1混合配比,给药方法均按0.1 mL/20 g的剂量对各品系小鼠进行腹腔注射给药,单用药物使用5只CD-1品系给药,混合药物采用6种品系,每组10只进行给药。结果单用速眠新Ⅱ小鼠全部死亡,单用舒泰50小鼠无法达到麻醉外科期。混合配比的方法,除了一只小鼠因保温不当而死亡,其余小鼠均能达到手术麻醉期,且正常苏醒。结论经过浓度换算,速眠新Ⅱ以10~25 mg/kg的浓度按1∶1的比例联合舒泰5010~25 mg/kg的浓度,在小鼠腹腔注射麻醉的实际使用中能达到理想效果,在小鼠实验麻醉中具有较强的应用推广价值。

Objective To explore the anesthesia effect of the new uncontrolled anesthetic drug Zoletil 50 and SumianxinⅡon SPF mice,hoping to find out a method that can achieve the ideal anesthesia effect for all strains of mice.Method Referring to the instructions of the drug,taking the dosage of dogs,cats and rabbits as reference,the mice of each strain were administered by intraperitoneal injection by using Sumianxin 11(30-fold dilution)alone;Zoletil 50(10-fold dilution)alone;mixed Sumianxin 11 and Sutai 50 both diluted by 10 times with 1∶1 mixing ratio;and mixed Sumianxin 11(20 times dilution)and Zoletil 50(5 times dilution)1∶1 mixing ratio,erspectively,and the administration dose was 0.1 mL/20 g.Five CD-1 strains were used to administer the single drug,and six strains were used to administer the mixed drug,with 10 mice in each group.Result All the mice with Sumianxin 11 died,and the mice with Zoletil 50 alone could not reach the surgical requirement of anesthesia.In both groups of the mixed Sumianxin 11 and Sutai 50,except for one mouse that died due to improper insulation,the rest of the mice could reach the requirement of surgical anesthesia and wake up normally.Conclusion Converting the result to the working concentration,SuMianxinⅡwith the concentration of 10-25 mg/kg combined with the concentration of 10-25 mg/kg of Sutai 50 at a ratio of 1∶1 can achieve the ideal effect in the actual use of intraperitoneal injection anesthesia in mice,suggesting a strong application value in mouse experimental anesthesia.