枳实麸炒前后对功能性消化不良大鼠内质网应激相关因子的影响

Effects of Aurantii Immaturus Fructus and its stir fried with bran product on endoplasmic reticulum stress-related factors in rats with functional dyspepsia

目的研究枳实麸炒前后对功能性消化不良大鼠胃组织内质网应激相关因子的影响。方法将60只SD大鼠随机分为空白组、模型组、2.7 mg·kg^(-1)多潘立酮组、9 g·kg^(-1)枳实生品组(RFAI)、9 g·kg^(-1)枳实麸品组(FFAIWB),采用“郭海军夹尾法”加以改进,刺激21 d复制功能性消化不良大鼠模型,造模结束后,灌胃给药14 d。采用定量法测定大鼠的肠推进率和胃排空率;HE染色法检测大鼠胃窦的病理情况;ELISA法检测血清中胃泌素的含量;RT-PCR法检测胃窦GRP78、IRE1、JNK的表达;Western Blot法检测Bcl-2、Bax的表达。结果与空白组比较,模型组大鼠的肠推进率、胃排空率和血清中胃泌素的含量显著下降;胃窦IRE1、GRP78、JNK mRNA的表达和Bax蛋白的表达明显增加,Bcl-2蛋白的表达下降;HE染色发现各组大鼠胃组织无明显器质性病变,但模型组大鼠的胃窦炎性浸润明显。与模型组比较,多潘立酮、枳实生品和枳实麸品组大鼠的消化不良症状有显著改善。结论枳实治疗模型大鼠与调控IRE1/GRP78/JNK信号通路有关,且麸炒后效果更佳。

OBJECTIVE To study the effects of Aurantii Immaturus Fructus(AIF)and its stir fried with bran product on endoplasmic reticulum stress-related factors of gastric tissues in rats with functional dyspepsia(FD).METHODS Sixty SD rats were randomly divided into blank group,model group,positive group(2.7 mg·kg^(-1) Domperidone),raw AIF group(9 g·kg^(-1) RAIF)and fried AIF with bran group(9 g·kg^(-1) FAIFWB).Rats were treated with the modified"Guo Haijun tail-clamping method"for 21 d to establish the FD rat model.After the model was built,all rats were given the corresponding solution by gavage for 14 d.The intestinal propulsion rate and gastric emptying rate were measured;Hematoxylin-Eosin(HE)staining to detect the pathology of rat stomach tissue;the levels of serum gastrin were detected by enzyme linked immunosorbent assay;the expressions of glucose regulatory protein 78(GRP78),inositol-requiring enzyme 1(IRE1)and c-Jun N-terminal kinase(JNK)were detected by reverse transcription polymerase chain reaction;the protein expressions of B-cell lymphoma-2(Bcl-2)-associated X(Bax)and Bcl-2 were detected by Western blot.RESULTS Compared with the blank group,the intestinal propulsion rate,gastric emptying rate,and the levels of serum gastrin of model group were significantly decreased,the mRNA expressions of IRE1,GRP78,JNK and protein expression of Bax in the gastric tissue increased;and protein expression of Bcl-2 decreased significantly.HE staining showed that the gastric tissues of all rats had no obvious organic lesions;however,the gastric tissues of model group had a large amount of inflammatory infiltration.Compared with model group,the FD symptoms of Domperidone,RAIF and FAIFWB rats were significantly improved.CONCLUSION AIF treats FD rats by regulating the IRE1/GRP78/JNK signaling pathway,and FIAFWB shows better effect.