纳米Cu@C复合材料对水绵生长的抑制作用

Inhibitory effects of nano-Cu@C composite material on the growth of Spirogyra sp.

为探究高效治理丝状绿藻水华的方法,研究了纳米Cu@C复合材料对丝状绿藻水绵(Spirogyra sp.)生长的抑制效果与作用机制以及对水环境的影响.结果显示,随着纳米Cu@C质量浓度从0增至40 mg·L^(-1),水绵的生物量和叶绿素a含量逐渐下降,呈明显的剂量-效应关系,生长抑制率最大可达60%;纳米Cu@C质量浓度增至50、60 mg·L^(-1),水绵的生长抑制率反而有所下降.纳米Cu@C对水绵细胞的可溶性蛋白含量、过氧化氢酶活性、过氧化物酶活性均呈低浓度促进,高浓度抑制,而对超氧化物歧化酶活性只产生不同程度的促进.各浓度纳米Cu@C暴露组水绵细胞的相对电导率和丙二醛含量均显著高于空白对照组(p<0.05).水绵胞内物质外流可能会造成水绵生长水体的总氮、总磷、高锰酸盐指数出现一定上升.纳米Cu@C的Cu^(2+)溶出浓度远低于饮用水安全标准,急性生物毒性较弱.综合来看,纳米Cu@C复合材料具有控制水绵孳生的应用潜力.

In order to explore an efficient way for controlling filamentous green algae blooms,the inhibitory effects and mechanisms of nano-Cu@C composite material on the growth of Spirogyra sp.were studied.The impact of nano-Cu@C on the aquatic environment was also investigated.The results showed that the biomass of Spirogyra and the content of chlorophyll a decreased as the concentration of nano-Cu@C increased from 0 to 40 mg·L^(-1),indicating an apparent dosage dependent relationship.The growth inhibition rate of Spirogyra by nano-Cu@C was up to 60%.When the concentration of nano-Cu@C increased to 50 and 60 mg·L^(-1),the growth inhibition rate of Spirogyra declined instead.Nano-Cu@C promoted the soluble protein content,catalase activity,and peroxidase activity of Spirogyra cells at low concentrations,while inhibited them at high concentrations.The superoxide dismutase activity of Spirogyra cells was promoted by nano Cu@C to different levels.The relative electric conductivity and malondialdehyde content of Spirogyra cells in each nano-Cu@C exposure group were significantly higher than those in the blank control group(p<0.05).The substances leaking from Spirogyra cells might increase the total nitrogen,total phosphorus,and permanganate index of the water where Spirogyra was grown.The concentration of Cu^(2+)caused by the dissolution of nano-Cu@C was much lower than that required by the drinking water standard,and the acute biological toxicity of nano-Cu@C was relatively mild.In conclusion,the nano-Cu@C composite material has the application potential in controlling the overgrowth of Spirogyra.