不同干燥方法对醋制延胡索物性及成分溶出的影响

Effects of Different Drying Methods on the Physical Property and Alkaloid Dissolution of Vinegar-Processed Corydalis Rhizoma

目的考察不同干燥方法(烘干、晒干、真空干燥、真空冷冻干燥)对醋制延胡索物性以及成分溶出的影响,为醋制延胡索干燥方法筛选提供理论依据。方法延胡索醋制后,分别进行烘干、晒干、真空干燥和真空冷冻干燥。比较复水率、折干率、硬度等物性参数,同时采用超高效液相色谱(UPLC)法测定醋制延胡索在不同时间段四氢非洲防己碱、原阿片碱、四氢黄连碱、非洲防己碱、延胡索乙素、盐酸小檗碱、盐酸巴马汀、去氢紫堇碱8种生物碱的溶出量,多指标结合化学计量学评价不同干燥方法对醋制延胡索饮片质量及成分溶出的影响。结果醋延胡索烘干后表面收缩,形成相对致密的结构,成分溶出较慢。冷冻干燥后疏松多孔,质地较轻且含水量少,成分溶出快。结论综合考虑成分含量、复水率、折干率、硬度等多指标,真空冷冻干燥醋制延胡索较其他干燥方法醋制延胡索有显著差异。真空冷冻干燥法处理的醋制延胡索饮片在加水回流提取20 min的成分溶出量明显高于另外3种干燥法处理的饮片,临床煎煮宜选用冷冻干燥法处理的醋制延胡索饮片。

Objective To investigate the effects of different drying methods(drying,sun drying,vacuum drying and freeze drying)on the physical property and alkaloid dissolution of Corydalis rhizoma processed with vinegar,and to provide a theoretical basis for the selection of drying methods of Corydalis rhizoma processed with vinegar.Methods After preparing Corydalis rhizoma processed with vinegar,heat drying,sun drying,vacuum drying,and vacuum freeze drying were carried out respectively.The physical property parameters such as rehydration rate,drying rate and hardness were compared.At the same time,the dissolution of tetrahydroclofenac,protopine,tetrahydrocoptimetine,tetrandrine,tetrahydropalmatine,berberine hydrochloride,palmatinehydrochloride,dehydroviodecanine were measured by UPLC method.Multi-index combined with chemometrics to evaluate the effects of different drying methods on the quality and component dissolution of Corydalis rhizoma processed with vinegar.Results After drying,the surface of the medicinal materials shrinks,forming a relatively dense structure,and the ingredients dissolve slowly.After freeze-drying,the medicinal materials are loose and porous,light in texture and low in water content,and the ingredients dissolve quickly.Conclusion Considering multiple indicators such as physical parameters and internal components,the freeze-dried vinegar-processed Corydalis rhizoma is significantly different from other drying methods.In the first 20 minutes,the dissolution rate of the components after the freeze-drying method was significantly higher than that of the other three methods.According to the actual clinical decoction,freeze-drying method should be used.