超高效液相色谱-串联质谱法快速检测白酒与配制酒中4种天然甜味剂

Determination of 4 natural sweetener compounds in Chinese baijiu and blended liquor by ultra performance liquid chromatography triple quadrupole mass spectrometry

目的:建立一种快速检测白酒和配制酒中4种天然甜味剂化合物方法。方法:样品经超声稀释定容,利用超高效液相色谱—质谱法进行定性和定量分析。结果:在10.0~200.0 ng/mL线性范围内,4种甜味剂的相关系数R2≥0.997。4种甜味剂检出限为2.88~28.5μg/kg,定量限(LOQ)为9.60~95.2μg/kg。4种甜味剂的加标回收率在84.4%~119%之间,相对标准偏差RSD在2.60%~10.0%之间。结论:该方法简单、高效、准确度高,可作为白酒和配制酒中甜菊糖苷、甜菊双糖苷、甘草酸和甘草次酸的定量检验方法。

Objective:A method for rapid determination of 4 natural sweetener compounds in Chinese baijiu and blended liquor was established.Methods:The samples were diluted to volume by ultrasound and analyzed qualitatively and quantitatively by UPLC-MS/MS.Result:The correlation coefficient R2 of the 4 sweeteners was≥0.997 in the linear range of 10.0~200.0 ng/mL.The limits of detection(LOD)of the method were from 2.88 to 28.5μg/kg and the limits of quantification(LOQ)were from 9.60 to 95.2μg/kg.The average recoveries of the 4 natural sweetener compounds were in the range from 84.4%to 119%,with RSD ranged within 2.60%~10.0%.Conclusion:The method was simple,efficient and accurate.It can be used for the quantitative confirmation of stevioside,steviobioside,glycyrrhizic acid and glycyrrhetinic acid in Chinese baijiu and blended liquor.