摘要
目的:建立一种快速检测白酒和配制酒中4种天然甜味剂化合物方法。方法:样品经超声稀释定容,利用超高效液相色谱—质谱法进行定性和定量分析。结果:在10.0~200.0 ng/mL线性范围内,4种甜味剂的相关系数R2≥0.997。4种甜味剂检出限为2.88~28.5μg/kg,定量限(LOQ)为9.60~95.2μg/kg。4种甜味剂的加标回收率在84.4%~119%之间,相对标准偏差RSD在2.60%~10.0%之间。结论:该方法简单、高效、准确度高,可作为白酒和配制酒中甜菊糖苷、甜菊双糖苷、甘草酸和甘草次酸的定量检验方法。
Objective:A method for rapid determination of 4 natural sweetener compounds in Chinese baijiu and blended liquor was established.Methods:The samples were diluted to volume by ultrasound and analyzed qualitatively and quantitatively by UPLC-MS/MS.Result:The correlation coefficient R2 of the 4 sweeteners was≥0.997 in the linear range of 10.0~200.0 ng/mL.The limits of detection(LOD)of the method were from 2.88 to 28.5μg/kg and the limits of quantification(LOQ)were from 9.60 to 95.2μg/kg.The average recoveries of the 4 natural sweetener compounds were in the range from 84.4%to 119%,with RSD ranged within 2.60%~10.0%.Conclusion:The method was simple,efficient and accurate.It can be used for the quantitative confirmation of stevioside,steviobioside,glycyrrhizic acid and glycyrrhetinic acid in Chinese baijiu and blended liquor.
作者
李涛
周艳华
向俊
汪霞丽
袁列江
LI Tao;ZHOU Yanhua;XIANG Jun;WANG Xiali;YUAN Liejiang(Hunan Provincial Institute of Product and Goods Quality Inspection Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning,Changsha 410011;Changsha Environmental Protection College,Changsha 410004)
出处
《中国食品添加剂》
CAS
北大核心
2023年第6期310-314,共5页
China Food Additives
基金
湖南省科技创新平台与人才计划项目(编号:2019TP1058)
湖南省市场监督管理局科技计划项目(2021KJJH46)。