辣椒WOX基因家族的鉴定及表达分析

Identification and analysis of WOX gene family in pepper

利用辣椒的全基因组数据从辣椒中鉴定出了10个WOX基因,并按照其在染色体上的分布顺序命名为CaWOX1、CaWOX2、 CaWOX3、…、CaWOX10,对它们的理化性质、染色体定位、与番茄的进化关系、蛋白保守基序、组织表达水平及在高温(42℃)、低温(10℃)胁迫下的表达水平进行分析。结果表明:辣椒WOX基因家族各成员的理化性质差异较大,10个家族成员编码的氨基酸长度为127~318 aa,蛋白相对分子质量为14 740~36 070,开放阅读框长度为384~957 bp,蛋白理论等电点(PI)为5.66~10.01;在染色体上的分布较为均匀,大部分分布在染色体的两端;系统进化树分析表明,辣椒WOX基因家族可分为现代、中间、古代3支,与番茄进化关系一致;蛋白保守基序显示,除CaWOX3外,其余蛋白都含有Motif1与Motif2,且二者总是紧密相连出现;组织表达水平分析表明,除了部分CaWOXs具有特异性表达模式外,大部分成员在组织中不表达或弱表达;高温、低温处理能够刺激或抑制WOX基因家族成员的表达。

In this study,10 WOX genes were identified from pepper by using the whole genome data of pepper and were named as CaWOX1,CaWOX2,CaWOX3,...,CaWOX10 according to their distribution order on the chromosomes.The physical and chemical properties,chromosome location,evolutionary relationship with tomato,protein conserved motif,tissue expression level and expression level under high and low temperature stress were analyzed.The results showed that the physical and chemical properties of each member were quite different.The lengths of amino acids encoded by the 10 genes were 127-318 aa,the relative molecular masses of the proteins were 14740-36070,the open reading frame lengths were 384-957 bp,and the theoretical isoelectric points of the proteins were 5.66-10.01.The distribution on the chromosome were more uniform,mainly localizing at the both ends of the chromosome.Phylogenetic tree analysis showed that the WOX gene could be divided into three branches:modern,middle and ancient,which was consistent with the evolutionary development of tomato.Protein conserved motifs showed that except for CaWOX3,the proteins contained Motif1 and Motif2,which were always closely linked.Tissue expression level analysis showed that most members were not expressed or weakly expressed in tissues except for some CaWOXs with specific expression patterns.High temperature and low temperature treatment could stimulate or inhibit the expression of WOX gene family members.