氢气对HaCaT角质形成细胞增殖及炎症因子表达的影响

Effects of hydrogen on proliferation of HaCaT keratinocytes and expression of inflammatory factors

目的:明确氢气(H2)对银屑病角质形成细胞模型增殖及炎症因子表达的影响,为探索氢气治疗银屑病的可能机制提供参考。方法:采用M5(含IL-17A、IL-22、肿瘤抑制素M、IL-lα和TNF-α)诱导HaCaT角质形成细胞过度增殖,并通过氢气细胞培养箱进行氢气干预。将HaCaT细胞分为Control组(无任何干预)、Model组(2.5 ng/mL M5处理)、H2组(2.5 ng/mL M5、25%浓度的氢气处理)、Acitretin组(2.5 ng/mL M5、10μmol/L的阿维A处理作为阳性对照),72小时后CCK-8法对细胞增殖进行测定,qRT-PCR检测细胞过度增殖标记因子KRT6及炎症因子IL-1β、IL-6、TNF-α的mRNA表达水平。结果:CCK-8法检测结果显示不同浓度氢气对正常细胞生长无影响,25%浓度的氢气干预能显著抑制M5诱导的细胞增殖,降低KRT6及IL-1β、IL-6、TNF-α的mRNA表达。结论:氢气干预能够抑制M5诱导的HaCaT细胞的过度增殖,降低炎症因子的表达。

Objective:To determine the effect of hydrogen(H 2)on the hyperproliferation of HaCaT keratinocytes and the expression of inflammatory factors,and to explore the possible mechanism of hydrogen in the treatment of psoriasis.Methods:Hyperproliferation of HaCaT keratinocytes was induced by M5(IL-17A,IL-22,tumor inhibin-M,IL-1αAnd TNF-α)and hydrogen intervention was conducted through hydrogen cell incubator.HaCaT cells were divided into Control group(without any intervention),Model group(treated by 2.5 ng/mL M5),H 2 group(treated by 2.5 ng/mL M5 and 25%hydrogen treatment),and Acitretin group(treated by 2.5 ng/mL M5,10μMol/L of acitretin A),after 72 hours,the cell viability was determined by CCK-8 method,and the cell proliferation marker KRT6 and inflammatory factor IL-1β,IL-6,TNF-αmRNA expression level were detected by qRT-PCR.Results:CCK-8 assay showed that different concentrations of hydrogen had no effect on normal cell growth.Hydrogen intervention at 25%concentration can significantly inhibit the enhancement of cell viability induced by M5,and reduced the level of KRT6 and IL-1β,IL-6,and NF-αmRNA expression.Conclusion:Hydrogen intervention can inhibit the over proliferation of HaCaT cells induced by M5 and reduce the expression of inflammatory factors.