摘要
Liquid biopsy is a technology that exhibits potential to detect cancer early,monitor therapies,and predict cancer prognosis due to its unique characteristics,including noninvasive sampling and real-time analysis.Circulating tumor cells(CTCs)and extracellular vesicles(EVs)are two important components of circulating targets,carrying substantial disease-related molecular information and playing a key role in liquid biopsy.Aptamers are single-stranded oligonucleotides with superior affinity and specificity,and they can bind to targets by folding into unique tertiary structures.Aptamer-based microfluidic platforms offer new ways to enhance the purity and capture efficiency of CTCs and EVs by combining the advantages of microfluidic chips as isolation platforms and aptamers as recognition tools.In this review,we first briefly introduce some new strategies for aptamer discovery based on traditional and aptamer-based microfluidic approaches.Then,we subsequently summarize the progress of aptamer-based microfluidics for CTC and EV detection.Finally,we offer an outlook on the future directional challenges of aptamer-based microfluidics for circulating targets in clinical applications.
基金
This work was supported by the National Natural Science Foundation of China(Grant Nos.:82003710 and 82173808)
the Natural Science Foundation of Guangdong Province(Grant Nos.:2020A1515010075 and 2021B1515020100)
the Project of Educational Commission of Guangdong Province(Grant No.:2021ZDZX2012)
the Guangzhou Basic and Applied Basic Research Project(Grant No.:2023A04J1163)
the National Key Clinical Specialty Construction Project(Clinical Pharmacy)
High-Level Clinical Key Specialty(Clinical Pharmacy)in Guangdong Province,China.
