摘要
β-乳球蛋白(β-lactoglobulin,BLG)可诱发严重的牛乳过敏反应,通过超声波辅助木犀草素(luteolin,LUT)对BLG进行处理,探究其结构和致敏性的变化规律。通过傅里叶变换红外光谱、圆二色光谱、紫外吸收光谱、荧光光谱等技术研究一次或二次超声波辅助LUT处理对BLG结构的影响;采用酶联免疫吸附测试、细胞实验对其致敏性变化进行评价。结果表明:超声波处理前后的BLG与LUT之间的主要作用力为疏水相互作用,超声波辅助LUT处理显著改变了BLG的二级结构和三级结构,降低了BLG的免疫球蛋白E结合能力以及KU812细胞中白细胞介素-6、白细胞介素-4和组胺的释放能力。因此,超声波辅助LUT处理通过改变BLG的构象表位降低其致敏性,二次超声波辅助LUT处理对BLG的降敏效果最好。
Bovineβ-Lactoglobulin(BLG)induces severe cow’s milk allergy.This research investigated the effect of ultrasound-assisted luteolin(LUT)treatment on the structure and allergenicity of BLG.The structure of the treated BLG was characterized by Fourier transform infrared(FTIR)spectroscopy,circular dichroism(CD)spectroscopy,ultraviolet(UV)absorption spectroscopy and fluorescence spectroscopy,and its allergenicity was evaluated by enzyme-linked immunosorbent assay(ELISA)and cell experiments.It was found that the major interaction force between BLG and LUT was hydrophobic interaction both before and after ultrasonic treatment.Ultrasound-assisted LUT treatment significantly altered the secondary and tertiary structures of BLG,and reduced the IgE-binding ability and the release of interleukin-6,interleukin-4 and histamine from KU812 cells.Therefore,ultrasound-assisted LUT treatment changes the conformational epitope of BLG,reducing its allergenicity,and the best effect can be achieved by secondary ultrasound-assisted LUT treatment.
作者
王梯梯
王阳
邹琳
刘俊
邵艳红
涂宗财
WANG Titi;WANG Yang;ZOU Lin;LIU Jun;SHAO Yanhong;TU Zongcai(National R&D Center for Freshwater Fish Processing,College of Life Sciences,Jiangxi Normal University,Nanchang 330022,China;State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第11期48-56,共9页
Food Science
基金
国家自然科学基金地区科学基金项目(31960457)
江西省自然科学基金青年基金项目(20202BABL215027)
江西省技术创新引导类计划项目(20212AEI91001)。
关键词
超声
木犀草素
Β-乳球蛋白
结构
致敏性
ultrasound
luteolin,β-lactoglobulin
structure
allergenicity
