茶籽皂苷联合有氧运动改善高脂饮食诱导的肥胖小鼠脂代谢及氧化应激水平

Tea Seed Saponin Combined with Aerobic Exercise Ameliorated Lipid Metabolism and Oxidative Stress in High-Fat Diet-Fed Mice

目的:探究茶籽皂苷(tea seed saponin,TSS)联合有氧运动(aerobic exercise,AE)对高脂饮食(high-fat diet,HFD)喂养小鼠脂代谢与氧化应激水平的改善作用。方法:随机将小鼠分为正常组和造模组,正常组饲喂普通维持饲料,造模组饲喂HFD建立肥胖模型,造模成功后分为HFD组、HFD+TSS组、HFD+AE组和HFD+TSS-AE组。试剂盒法检测小鼠血清脂代谢和氧化应激水平;苏木精-伊红染色观察肝脏的脂肪变性程度;蛋白免疫印迹法检测肝脏腺苷酸活化蛋白激酶(adenosine 5’-monophosphate(AMP)-activated protein kinase,AMPK)、p-AMPK、沉默信息调节因子1(silent information regulator 1,SIRT1)、过氧化物酶体增殖物激活受体γ辅激活因子1α(peroxisome proliferator-activated receptorγcoactlvator 1α,PGC-1α)及骨骼肌过氧化物酶体增殖物激活受体γ(peroxisome proliferators-activated receptorsγ,PPARγ)的表达。结果:TSS联合AE干预后,HFD小鼠体质量和皮下、附睾、肾周及腹部等处脂肪指数显著降低(P<0.05);血脂紊乱改善,HFD+TSS-AE组小鼠血清总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平较HFD组分别显著降低27.80%、75.15%、78.36%(P<0.05);与HFD组相比,HFD+TSS组、HFD+AE组和HFD+TSS-AE组小鼠肝脏脂肪变性程度得到显著改善,肝脏内p-AMPK表达水平分别提高了64.19%、1.12倍和1.64倍,SIRT1表达水平分别提高了47.01%、1.48%和67.02%,PGC-1α表达水平分别提高了1.03倍、62.93%和1.10倍,以及骨骼肌PPARγ表达水平分别提高了64.08%、1.12倍和2.34倍。此外,TSS联合AE干预还使HFD小鼠血清和肝脏SOD和GSH水平升高,并降低MDA水平。结论:TSS联合AE干预小鼠可减少体内脂肪堆积,改善HFD引起的脂质代谢紊乱和氧化应激状态。

Objective:The effect of tea seed saponin(TSS)combined with aerobic exercise(AE)on lipid metabolism and oxidative stress in mice on a high-fat diet(HFD)was investigated.Methods:Mice were randomly divided into a normal group and a model group.The normal group was fed on a maintenance diet and the model group on the HFD to establish an obesity model.After successful modeling,the mice were further divided into an HFD group,an HFD+TSS group,an HFD+AE group and an HFD+TSS-AE group.The levels of serum lipid metabolism and oxidative stress were determined by commercial kits.Hepatic steatosis was observed by hematoxylin-eosin(HE)staining.The protein levels of adenosine 5’-monophosphate(AMP)-activated protein kinase(AMPK),phosphorylated AMPK(p-AMPK),silent information regulator 1(SIRT1),peroxisome proliferator-activated receptorγcoactlvator 1α(PGC-1α)and peroxisome proliferators-activated receptorsγ(PPAR-γ)were detected by Western blot.Results:TSS combined with AE treatment significantly reduced body mass and subcutaneous,epididymal,perirenal and abdominal fat indexes in HFD mice(P<0.05)and improved blood lipid disorders.The serum levels of total cholesterol(TC),triglyceride(TG)and low-density lipoprotein cholesterol(LDL-C)were,respectively,reduced by 27.80%,75.15%,78.36%in the HFD+TSS+AE group compared with the HFD group.Hepatic steatosis was significantly improved in the HFD+TSS,HFD+AE and HFD+TSS-AE groups in comparison with the HFD group.The expression level of hepatic p-AMPK was increased by 64.19%,2.12 times and 2.64 times,SIRT1 by 47.01%,1.48%and 67.02%,PGC-1αby 2.03 times,62.93%and 2.10 times,and PPAR-γin skeletal muscle by 64.08%,2.12 times and 3.34 times,respectively.After treated by TSS and AE,the serum and liver levels of superoxide dismutase(SOD)and glutathione(GSH)were increased in HFD mice,and serum malondialdehyde(MDA)levels were decreased.Conclusion:TSS combined with AE can reduce fat accumulation and improve lipid metabolism disorders and oxidative stress induced by HFD in mice.