香茅醛对指状青霉孢子麦角固醇合成的影响

Effect of Citronellal on Ergosterol Synthesis in Penicillium digitatum Spores

探讨了香茅醛对指状青霉抑霉唑敏感(Pds01)和抗性(Pdw03)菌株孢子萌发过程中麦角固醇合成的影响。结果表明:不同含量香茅醛能显著抑制Pds01和Pdw03孢子萌发,半抑制浓度分别为0.8μL/mL和3.2μL/mL。经半抑制浓度香茅醛处理不同时间后,Pds01和Pdw03孢子麦角固醇含量显著降低,羊毛固醇含量显著升高;麦角固醇合成关键基因表达受到显著影响,Pds01的ERG9基因上调表达峰值早于Pdw03,两者ERG3基因的表达量皆显著下调。本研究表明香茅醛通过抑制ERG3的表达降低Pds01和Pdw03孢子麦角固醇合成,进而影响孢子萌发,可为柑橘采后病害绿色防腐保鲜剂的开发提供理论依据。

In this study,the effect of citronellal on the synthesis of ergosterol in the spores of imazalil-sensitive(Pds01)and imazalil-resistant(Pdw03)strains of Penicillium digitatum was investigated.The results showed that citronellal significantly inhibited the spore germination of Pds01 and Pdw03 with half maximal inhibitory concentration(IC50)of 0.8 and 3.2μL/mL,respectively.Citronellal at its IC50 significantly decreased and increased the contents of ergosterol and lanosterol in Pds01 and Pdw03 spores,respectively.The expression of key genes involved in ergosterol synthesis was significantly affected.The up-regulated expression of the ERG9 gene in Pds01 peaked earlier than that in Pdw03,and the expression level of ERG3 was significantly down-regulated in both strains.This study showed that citronellal reduced ergosterol synthesis in Pds01 and Pdw03 spores by inhibiting ERG3 expression,thereby affecting spore germination,which could provide a theoretical basis for the development of green preservatives for postharvest citrus diseases.