大鼠改良脊髓栓系综合征模型的建立及评估

Establishment and evaluation of modified tethered spinal cord model in rats

目的探讨SD大鼠改良脊髓栓系综合征模型的有效性和可靠性。方法2020年9月在新疆医科大学第一附属医院选取清洁级SD大鼠60只,按照体重随机分为实验组和对照组,每组各30只,实验组采用改良慢性牵拉法建立大鼠脊髓栓系综合征模型。分别检测大鼠后肢行为学功能、膀胱功能、运动诱发电位及脊髓病理生理变化,应用SPSS 20.0软件进行分析。结果实验组大鼠术后7 d残余尿量为(1.94±0.23)ml,明显高于对照组[(0.18±0.16)ml,t=3.886,P<0.05],术后30 d实验组大鼠出现严重的膀胱功能障碍[(2.92±0.32)ml],明显高于对照组[(0.19±0.21)ml,t=7.542,P<0.05]。同时,与对照组比较,实验组大鼠Tarlov评分降低,运动诱发电位(MEP)中的P波潜伏期延长、波幅下降(χ^(2)=4.876,t=3.468,P<0.05);病理检测发现,脊髓末端组织观察到不同程度的神经细胞坏死、胞浆内尼氏体淡然,数量明显减少并分布不均匀。结论SD大鼠改良脊髓栓系综合征模型为脊髓栓系综合征的基础及临床研究提供更有效的模型。

Objective To explore the validity and reliability of the modified spinal cord embolism syndrome model in SD rats.Methods In September 2020,60 clean SD rats were selected from the First Affiliated Hospital of Xinjiang Medical University,and the 60 rats were randomly divided into experimental and control groups.30 SD rats were used in the experimental group and 30 SD rats in the control group,and the rat spinal cord embolism syndrome model was established by the modified chronic pulling method in the experimental group,and only laminectomy was performed in the control group.The behavioral function of the hind limbs,bladder function,motor evoked potentials and pathophysiological changes of the spinal cord were examined separately and analyzed by SPSS 20.O software.Results The residual urine volume of rats in the experimental group was(1.94±0.23)ml at 7 d postoperatively,which was significantly higher than that in the control group[(0.18±0.16)ml,t=3.886,P<0.05],and the rats in the experimental group showed severe bladder dysfunction at 30 d postoperatively(2.92±0.32)ml,which was significantly higher than that in the control group[(0.19±0.21)ml,t=7.542,P<0.05].At the same time,com-pared to the control group,the rats in the experimental group had lower Tarlov scores,longer P-wave laten-cies and lower wave amplitudes in motor evoked potentials(MEP)(χ^(2)=4.876,t=3.468,P<0.05);pathological examination revealed that different degrees of neuronal cell necrosis,intracytoplasmic nictita-ting bodies were observed in the spinal cord terminal tissues,which were significantly reduced in number and unevenly distributed.Conclusion The SD rat modified spinal cord embolism syndrome model pro-vides a more effective model for basic and clinical studies of spinal cord embolism syndrome.