绞股蓝总苷对小鼠缺血性脑卒中的脑保护作用及细胞凋亡影响

Effects of gypenosides on brain protection and cell apoptosis after cerebral ischemic stroke in mice

目的探讨绞股蓝总苷对小鼠缺血性脑卒中(CIS)的影响。方法健康雄性C57小鼠,随机分为假手术组,溶剂组,用药组,每组6只,采用线栓法建立小鼠大脑中动脉阻塞动物模型,用药组:小鼠大脑中动脉闭塞后2h绞股蓝总苷200mg·kg^(-1)腹腔注射,假手术组:小鼠假手术后2h生理盐水腹腔注射;溶剂组:小鼠大脑中动脉闭塞后2h生理盐水腹腔注射。术后24h,对小鼠进行神经功能评分,脑梗死体积测定,脑组织含水量测定及伊文思蓝染色,检测Bax、Bcl-2在小鼠脑组织中表达。结果应用绞股蓝总苷的小鼠与溶剂组小鼠相比,神经功能评分明显降低(P<0.05);脑梗死体积明显减小(P<0.05);与假手术组小鼠相比,溶剂组小鼠脑组织的含水量明显增高,而应用绞股蓝总苷的小鼠脑组织的含水量在24h明显降低(P<0.05);溶剂组小鼠血-脑脊液屏障(BCFB)完整性严重破坏,用药组小鼠脑组织伊文思蓝渗出明显减少(P<0.05)。与假手术组相比,溶剂组小鼠Bax及Bcl-2的mRNA及蛋白水平明显升高,应用绞股蓝总苷后可下调Bax及上调Bcl-2的表达。结论绞股蓝总苷在CIS急性期降低神经功能缺损评分,减少脑梗死体积,减轻伊文思蓝渗出,具有脑保护作用,机制可能与抗凋亡有关。

Objective To explore the effect of gypenosides on cerebral ischemic stroke(CIS)in mice.Methods Healthy male C57 mice were randomly divided sham group,vehicle group and drug group,and 6 mice in each group.The middle cerebral artery occlusion model was established by suture method.The drup group:gypenosides 200mg·kg^(-1)was intraperitoneally injected 2 hours after middle cerebral artery occlusion in mice.Sham Group:normal saline was intraperitoneally injected 2 hours after sham operation in mice.Vehicle group:normal saline was intraperitoneally injected 2 hours after middle cerebral artery occlusion in mice.At 24h postoperatively,the mice were assessed for neurological deficit,infarct volume,brain water content and Evans blue staining by the single blind method.The mRNA and protein levels of Bax and Bcl-2 were detected.Results The neurological deficit in the mice treated with gypenosides was significantly lower than that in the vehicle group(P<0.05).The cerebral infarction volume in the vehicle group was larger than drug group,and the change was significantly(P<0.05).Compared with sham group,the water content of brain tissue in vehicle group was higher,while the water content of brain tissue in mice treated with gypenosides was significantly decreased after 24h(P<0.05).The blood brain barrier of vehicle mice was seriously damaged,and the exudation of evans blue in gypenosides treated mice decreased significantly(P<0.05).Compared with the sham group,the mRNA and protein levels of Bax and Bcl-2 in vehicle group were increased,and the expression of Bax was down-regulated and the expression of Bcl-2 was up-regulated after the application of gypenosides.Conclusion In the acute stage of CIS,gypenosides can reduce the neurological deficit score,reduce the volume of cerebral infarction and alleviate Evans blue exudation,which has brain protective effect,and the mechanism may be related to anti-apoptosis.