CRISPR系统在白假丝酵母菌基因功能研究中的应用

CRISPR system in the study of gene function of Candida albicans

成簇的间隔短回文重复(CRISPR)系统作为一种有效的基因编辑策略,已成为多种生物系统基因功能研究的通用工具。真菌感染是导致人类疾病的重要原因,白假丝酵母菌作为临床最常见的病原真菌,其基因功能的揭示是探寻新的治疗策略和药物靶点的关键。本文回顾了CRISPR系统在白假丝酵母菌中从应用到优化,从基因敲除到表达调控的发展历程,以期通过对白假丝酵母菌致病力及耐药机制的研究为新药物靶点的探寻开辟道路。

As an effective gene editing strategy,clustered regularly interspaced short palindromic repeats(CRISPR)system has become a general tool for gene function research in many biological systems.Fungal infection is an important cause of human diseases.As the most common clinical pathogenic fungi,the revelation of the gene function of Candida albicans is the key to new treatment strategies and drug targets.This paper reviewed the development of CRISPR system in Candida albicans from application to optimization,from gene knockout to expression regulation,to pave the way for the exploration of new drug targets via pathogenicity and drug resistance mechanism of Candida albicans.