非抑制型离子色谱法测碳酸镧和碳酸镧咀嚼片中镧的含量

Determination of lanthanum in lanthanum carbonate and lanthanum carbonate chewable tablets by non-suppressive conductance detection ion chromatography

目的建立非抑制型离子色谱法测定碳酸镧和碳酸镧咀嚼片中镧的含量。方法采用离子色谱法,色谱柱为Thermo Dionex IonPac SCS1(4 mm×250 mm)交换柱和Dionex IonPac SCG1(4 mm×50 mm)保护柱,检测器为非抑制电导检测器,电导检测池温度为35℃,柱温为30℃,进样量为25μL,淋洗液为6 mmol·L^(-1)硝酸HNO_(3)溶液,流速为1.0 mL·min^(-1)。结果镧在10~80μg·mL^(-1)内与峰面积线性关系良好,相关系数为0.9994,检测限为0.15μg·mL^(-1),定量限为0.5μg·mL^(-1),方法精密度好,溶液室温24 h内稳定。回收率在99.0%~100.0%,RSD小于1.6%。结论本方法测定碳酸镧咀嚼片及其原料药中镧的含量,专属性强,结果准确可靠。

Objective To determine the content of lanthanum in lanthanum carbonate and lanthanum carbonate chewable tablets by non-suppressive conductance detection ion chromatography(IC)method.Methods The determination was performed on Thermo Dionex IonPac SCS1(4 mm×250 mm)anion chromatography column and Dionex IonPac SCG1(4 mm×50 mm)guard column.The non-suppressive conductance detector was used.The detection tank temperature was 35℃;the column temperature was 30℃;and the injection volume was 25μL.HNO_(3) solution(6 mmol·L^(-1))was used as the isocratic elution and the flow rate was 1.0 mL·min^(-1).Results Lanthanum showed good linearity at 10~80μg·mL-1,the correlation coefficient was 0.9994,the detection limit was 0.15μg·mL^(-1)(S/N=3),and the quantification limit was 0.5μg·mL^(-1)(S/N=10).The precision was good,and the solution was stable for 24 h at room temperature.The recovery was 99.0%~100.0%,and the RSD lower than 1.6%(n=3).Conclusion The method to determine the content of lanthanum in lanthanum carbonate and lanthanum carbonate chewable tablets is sensitive and reproducible.