肺腺癌伴恶性胸腔积液患者胸水细胞蜡块KCa3.1的表达及其意义

Expression of KCa3.1 in pleural fluid cells of lung adenocarcinoma patients with malignant pleural effusion and its clinical significance

目的探讨肺腺癌(LUAD)伴恶性胸腔积液患者胸水细胞蜡块中电导钙激活钾离子通道蛋白(KCa3.1)的表达及其意义。方法利用GEPIA、Kaplan-Meier plotter数据库分析KCa3.1在肺腺癌组织中的表达情况及其与肿瘤分期、总生存期(OS)的关系。选取2019年6月1日至2021年6月30日绍兴市人民医院收治的LUAD伴恶性胸腔积液患者72例,应用免疫组化法检测胸水细胞蜡块KCa3.1表达,分析其与临床特征及预后的关系。结果生物信息学分析和临床资料分析提示,LUAD患者KCa3.1表达水平明显高于正常肺组织(P<0.05),KCa3.1高表达和低表达患者性别、年龄、吸烟情况、T分期、N分期、肺外转移情况、表皮生长因子受体基因突变情况比较差异均无统计学意义(均P>0.05),KCa3.1高表达患者OS低于低表达患者(P<0.05)。结论KCa3.1在LUAD患者中高表达,且与不良预后有关,提示KCa3.1可作为LUAD患者诊断和预后判断的潜在靶点。

Objective To investigate the expression of KCa3.1 in pleural effusion cells of lung adenocarcinoma(LUAD)patients with malignant pleural effusion and its clinical significance.Methods The expression of KCa3.1 in LUAD and its relationship with tumor stage and overall survival(OS)were analyzed using GEPIA,Kaplan-Meier plotter database.Seventytwo LUAD patients with malignant pleural effusion admitted to Shaoxing People's Hospital from June 2019 to June 2021 were enrolled.The expression of KCa3.1 in pleural fluid cell blocks was detected by immunohistochemical method,and its relationship with malignant clinical characteristics and prognosis of patients was analyzed.Results Bioinformatics analysis and clinical data indicated that the expression level of KCa3.1 in LUAD patients was significantly higher than that in normal lung tissues(P<0.05).The expression level of KCa3.1 was not correlated with sex,age,smoking status,T stage,N stage,extrapulmonary metastasis and EGFR mutation(all P>0.05).Patients with high expression of KCa3.1 had lower OS than those with low expression(P<0.05).Conclusion KCa3.1 is highly expressed in LUAD patients and is related to poor prognosis,suggesting that KCa3.1 can be a potential target for diagnosis and prognosis of LUAD patients.