清道夫受体B1基因启动子甲基化与冠心病的关联分析

Association Analysis Between Methylation of SCARB1 Gene Promoter and Coronary Heart Disease

目的 探讨清道夫受体B1(SCARB1)基因启动子区域甲基化与冠心病发病的关联性。方法 选取2018年12月至2020年5月在上海交通大学医学院附属仁济医院就诊的120例冠心病患者作为病例组,并随机选取同期健康体检人群中性别和年龄匹配的140名健康受试者作为对照组,进行病例对照研究。采用亚硫酸盐转化,扩增目标区域后采用高通量测序对基因组进行甲基化检测,分析两组SCARB1基因启动子区域CpG位点的甲基化差异。结果 病例组SCARB1+67基因的甲基化水平显著高于对照组(P<0.001),SCARB1+134基因的甲基化水平显著低于对照组(P<0.001),且在男性(P均<0.001)、女性(P均<0.001)冠心病患者中与对照组比较差异有统计学意义。病例组SCARB1基因的平均甲基化水平显著低于对照组[(80.27±2.14)%比(81.11±1.27)%;P=0.006],且只在男性冠心病患者中差异有统计学意义(P=0.002)。结论 SCARB1基因的甲基化水平与冠心病的发病存在关联,可能参与其发生发展过程。

Objective To explore the relationship between scavenger receptor class B member 1(SCARB1)gene promoter methylation and the pathogenesis of coronary artery disease.Methods A total of 120 patients with coronary heart disease treated in Renji Hospital affiliated to Shanghai Jiao Tong University School of Medicine from December 2018 to May 2020 were selected as the case group,while 140 gender and age matched healthy participants were randomly selected as the control group for a case-control study.The methylation status was detected by high-throughput target sequencing after bisulfite converting,and the methylation of CpG sites in the promoter region of SCARB1 gene was compared between the two groups.Results The case group showed higher methylation level of SCARB1+67 and lower methylation level of SCARB1+134 than the control group(both P<0.001),and the differences remained statistically significant in men(both P<0.001)and women(both P<0.001).The overall methylation level in the case group was lower than that in the control group[(80.27±2.14)%vs.(81.11±1.27)%;P=0.006],while this trend was statistically significant only in men(P=0.002).Conclusion The methylation of SCARB1 gene promotor is associated with the pathogenesis and may participate in the occurrence and development of coronary heart disease.