食品污水中异养硝化-好氧反硝化菌Bacillus sp.JG441的筛选鉴定及其脱氮特性

Screening,identification and denitrification characteristics of Bacillus sp.JG441 with heterotrophic nitrification and aerobic denitrification resistant from food industry wastewater

高氨氮、高盐、含有机物的食品工业污水处理难度大、周期长,添加高效硝化菌株可以大幅提高污水处理效率。本研究通过高盐培养基,从腌渍食品厂排放污水中筛选出一株对复杂环境有较强耐受性的新型高效异养硝化-好氧反硝化菌株JG441,经鉴定为Bacillus sp.,对其脱氮条件、异养硝化、好氧反硝化、极端环境耐受和脱氮通路进行研究。结果表明,菌株JG441可以利用(NH_(4))_(2)SO_(4)和KNO_(3)为氮源进行异养硝化和好氧反硝化;在NaCl浓度为30g·L^(-1),苯酚浓度为400mg·L^(-1)时,24h NH_(4)^(+)-N去除率可达99%,在NH_(4)^(+)-N浓度为500mg·L^(-1)时,24h NH_(4)^(+)-N去除率为57.4%。菌株JG441脱氮能力强,在高盐含氮和成分复杂的污水处理方面具有较好应用潜力。

The sewage treatment of food industry with high ammonia nitrogen,high salt and organic matter is difficult and long cycle.The addition of efficient nitrifying strains can greatly improve the efficiency of sewage treatment.A novel efficient heterotrophic nitrification-aerobic denitrification strain JG441 with high tolerance to complex environment was screened by high salt phenol medium from wastewater discharged from food factory.The strain was identified as Bacillus sp.and its optimum denitrification conditions,heterotrophic nitrification,aerobic denitrification,extreme environmental tolerance and denitrification pathway were studied.The results showed that strain JG441 could use ammonium sulfate and potassium nitrate as nitrogen sources for heterotrophic nitrification and aerobic denitrification.When the concentration of sodium chloride was 30g·L^(-1)and phenol was 400mg·L^(-1),the removal rate of NH_(4)^(+)-N reached 99%after 24h cultivation,and the removal rate of NH_(4)^(+)-N was 57.4%at 500mg·L^(-1)concentration of NH_(4)^(+)-N.Strain JG441 has a strong ability to remove nitrogen,and it has a high potential in application of high salt nitrogen and complex composition wastewater treatment.