基于代谢组学研究谷子SiYABBYs参与花发育过程中鼠李糖苷的生物合成

SiYABBYs Involved in Rhamnoside Biosynthesis During the Flower Development of Setaria italica,Based on Metabolomics

花器官发育是生殖发育的前提和基础,对小穗最终的形成具有决定性作用,YABBY转录因子在花器官的发育中具有重要作用,探究谷子YABBY家族成员的序列特征及表达模式,揭示其与谷子穗发育的关系,为阐明谷子YABBY基因家族的功能以及提高谷子产量奠定理论基础。通过对谷子YABBY基因家族进行全基因组鉴定,分析SiYABBYs的理化性质、组织表达模式,并与拟南芥和水稻YABBY蛋白序列比对进行功能预测。结果表明,共鉴定8个YABBY家族成员,编码150-303个氨基酸,在抽穗期和授粉期的穗中特异表达,可能参与谷子营养组织的发育和花器官的形成。对谷子抽穗期和授粉期的代谢组数据与SiYABBYs的表达水平进行相关性分析发现,SiYABBY1/SiYABBY5/SiYABBY7/SiYABBY8与鼠李糖苷代谢通路的酶基因紧密关联,表明促进授粉期穗中鼠李糖苷的积累,可能与谷子的成育率及结实率相关。

Flower organ is the premise and basis of reproductive development,and also plays a decisive role at the final stage of spikelet formation.YABBY transcription factors play an important role in the development of flower organs.In order to clarify the function of YABBY gene family and improve the yield of foxtail millet(Setaria italica),we studied the sequence characteristics and expression patterns of YABBY gene family members in the foxtail millet.We analyzed genome-wide identification of YABBY gene family in the foxtail millet,the physical and chemical properties,and tissue expression patterns of SiYABBYs gene.Also,we aligned YABBY sequences of the foxtail millet,Arabidopsis and rice(Oryza sativa)for functional prediction.The results showed that 8 YABBY family members in the foxtail millet,encoding 150-303 amino acids.And we found these SiYABBYs genes were specifically expressed in panicles at heading and pollination stages,which may be involved in the development of vegetative tissues and the formation of flower organs in the foxtail millet.The expressions of SiYABBYs were correlated with the metabolome data at heading and pollination stages of the foxtail millet.The results showed that SiYABBY1/SiYABBY5/SiYABBY7/SiYABBY8 were closely related to rhamnoside metabolic pathway.Also,the results indicated that rhamnoside accumulation in the panicles during the pollination might be related to the fertility and seed setting rate of the foxtail millet.