郑州地区宠物犬体内常见细菌的分离鉴定及药敏试验

Isolation, Identification and Antimicrobial SusceptibilityTest of Common Bacteria from Pet Dogs in Zhengzhou

为了解宠物犬体内常见细菌种类及其对临床常用抗生素的耐药情况,选取郑州市数家宠物医院对患病犬和健康犬分别进行样本采集。通过分离培养纯化,16S rRNA序列分析鉴定,获得大肠杆菌12株、葡萄球菌8株、肠球菌17株、肺炎克雷伯菌5株及沙门氏菌2株。采用微量肉汤稀释法测定7大类14种抗生素的最小抑菌浓度(MIC值),进行药物敏感性和多重耐药表型分析。结果表明,所有细菌总体对替加环素和黏菌素的敏感性较高,对其它抗菌药物的敏感性因细菌种类不同而产生差异。犬源细菌多重耐药现象较为普遍,本试验菌株的多重耐药率为59%(26/44),其中大肠杆菌、葡萄球菌和肺炎克雷伯菌的多重耐药率分别为19.2%、30.8%和7.7%。本试验初步探明了郑州地区宠物犬源常见细菌的药物敏感性及多重耐药情况,对宠物犬临床应用抗菌药具有指导意义。

In order to understand the common bacterial species in pet dogs and their resistance to clinically common antibiotics,samples from sick dogs and healthy dogs were collected from several pet hospitals in Zhengzhou city.12 strains of Escherichia coli,8 strains of Staphylococcus,17 strains of Enterococcus,5 strains of Klebsiella pneumoniae and 2 strains of Salmonella were isolated,purified and identified by 16S rRNA sequence analysis.Broth microdilution method was used to determine the minimum inhibitory concentration(MIC)of 14 antibiotics in 7 categories,and to analyze their antimicrobial susceptibility and multidrug resistance phenotype.The results showed that all bacteria were highly sensitive to tigecycline and colistin,and the sensitivity to other antibiotics varied with different bacterial species.Multiple drug resistance was common among canine-derived bacteria,and the multiple drug resistance rate of this test strain was 59%(26/44),among which that of Escherichia coli,Staphylococcus and Klebsiella pneumoniae were 19.2%,30.8%and 7.7%,respectively.This test preliminarily proved the antimicrobial susceptibility and multiple drug resistance of common bacteria from pet dogs in Zhengzhou city,which is of guiding significance for clinical application of antibiotics in pet dogs.