基于TCGA数据库分析HMGB1在结肠癌中的表达及临床意义

Expression and Clinical Significance of HMGB1 in Colon Cancer Based on TCGA Database

目的运用癌症基因组图谱(TCGA)数据库,探究高迁移率族蛋白B1(high mobility group box 1,HMGB1)在结肠癌组织样本和正常组织样本中的表达情况,挖掘其与结肠癌临床特征的相关性。方法运用生物信息学方法从TCGA数据库中获取结肠癌(COAD)患者HMGB1的表达数据及临床资料。运用R.4.2.2软件和Strawberry Perl 5.30.0.1软件分析HMGB1在结肠癌和正常组织样本之间的表达差异。运用R语言分析HMGB1表达量与临床资料的相关性,绘制Kaplan-Meier曲线进行生存分析,并利用单基因富集分析(ssGSEA)法富集HMGB1在结肠癌中所参与的信号通路。结果(1)HMGB1在14种癌症及正常组织间的表达差异有统计学意义(P<0.05)。(2)总计下载结肠癌组织样本476例、正常结肠组织样本41例。与正常组织样本相比,结肠癌组织样本中HMGB1表达量明显升高,差异具有统计学意义(P<0.001)。(3)HMGB1的表达与M分期和Stage分期相关。(4)HMGB1的表达与结肠癌患者的生存时间无明显相关。(5)ssGSEA结果显示,HMGB1主要参与RNA降解、Nod受体信号通路、半胱氨酸和蛋氨酸代谢、P53信号通路、错配修复、Wnt信号通路、ErbB信号通路、嘌呤代谢、MAPK信号通路、T细胞受体信号通路、DNA复制等多条通路。结论HMGB1在结肠癌中高表达,参与结肠癌发生发展的多条通路,与其恶性程度息息相关。HMGB1可作为判断结肠癌的标志物,并有望成为结肠癌靶向治疗的新方向。

Objective The Cancer Genome Atlas(TCGA)database was used to investigate the expression of high mobility group protein B1 in colon cancer tissue samples and normal tissue samples,and to explore its correla⁃tion with clinical characteristics of patients with colon cancer.Methods Hmgb1 expression and clinical data of co⁃lon cancer(COAD)patients were obtained from the TC GA database using bioinformatics techniques.Hmgb1 expres⁃sion differences in colorectal cancer and normal tissue samples were analyzed using r4.2.2 software and Straw Berry Perl 5.30.0.1 software.The correlation between HMGB1 expression levels and patient clinical data was analyzed u⁃sing the R language and the Kaplan-Meier curve was drawn.Single gene enrichment analysis(ssGSEA)accumula⁃ted HMGB1 signaling pathways in colorectal cancer.Results(1)There were significant differences in the expres⁃sion of HMGB1 in 14 cancers and normal tissues(P<0.05).(2)A total of 476 colon cancer tissue samples and 41 normal colon tissue samples were downloaded.Compared with normal tissue samples,the expression level of HMGB1 in colon cancer tissue samples was significantly increased,and the difference was statistically significant(P<0.001).(3)Expression and M staging of HMGB1 in colon cancer tissue samples and Stage staging(P<0.05).(4)The expression of HMGB1 was not correlated with the survival time of colon cancer patients(P>0.05).(5)ssG⁃SEA single gene enrichment analysis showed that HMGB1 was mainly involved in RNA degradation,Nod receptor signaling pathway,cysteine and methionine metabolism,P53 signaling pathway,mismatch repair,Wnt signaling path⁃way,ErbB signaling pathway,purine metabolism,MAPK signaling pathway,T cell receptor signaling pathway,DNA replication and other pathways.Conclusion HMGB1 is highly expressed in patients with colon cancer,which is closely related to the degree of malignancy and involved in various signaling pathways to promote the occurrence of colon cancer.HMGB1 can be used as a marker for colon cancer and is expected to be a new direction of targeted therapy for colon cancer.