聚茜素红-羧基化多壁碳纳米管修饰电极测定花生壳中木犀草素的含量研究

Determination of Luteolin in Peanut Shell by Poly Alizarin Red-Carboxylated Multi-walled Carbon Nanotube Modified Electrode

基于羧基化多壁碳纳米管(C-MWCNT),利用聚茜素红(PAR)的电催化特性在玻碳电极(GCE)上电聚合制备了PAR/C-MWCNT/GCE电化学修饰电极,并对花生壳中木犀草素(Lut)的含量测定进行了方法建立和实际应用。采用SEM、XRD对电极表面修饰剂的形貌和组成进行表征。通过循环伏安法和差分脉冲伏安法探究了Lut在电极上的电化学行为。结果表明,pH 8.5的磷酸盐缓冲溶液中,Lut在该修饰电极上的响应电流是裸电极的3倍,具有良好的分析性能,检测Lut的线性范围为1~100μmol/L,线性方程为y=0.372 28x-0.016 75(R^(2)=0.993 3)。该电极具有制备简单、选择性好和线性范围宽的特点,测得花生壳中Lut的含量为8.514μmol/L,加标回收率为91%~98%,相对标准偏差为2.62%。

By taking advantage of the electrocatalytic property of poly alizarin red(PAR),a poly alizarin red(PAR)/carboxylated multi-walled carbon nanotube(C-MWCNT)modified glassy carbon electrode(GCE)was fabricated for the determination of luteolin(Lut)in peanut shell.The morphology and composition of the surface of modified electrode were characterized by SEM and XRD analyses.The electrochemical behavior of Lut on the modified electrode was explored by cyclic voltammetry and differential pulse voltammetry.The results showed that the current of Lut on the modified electrode was three times as that of the bare electrode in the phosphate buffer solution with pH 8.5.Under optimized conditions,a good linear relationship(y=0.37228 x-0.01675,R^(2)=0.9933)was established with a concentration of Lut covering from 1 to 100μmol/L.The PAR/C-MWCNT/GCE is easy to prepare,and the as-proposed method exhibits good selectivity and broad linear range for the detection of Lut.The PAR/C-MWCNT/GCE based assay was applied for the determination of Lut with the result was 8.514μmol/L in peanut shells.The recoveries ranged from 91%to 98%with the relative standard deviation less than 2.62%.