摘要
目的:建立了RP-HPLC法测定眩晕宁颗粒制剂中异绿原酸A等3种成分含量的方法。方法:CapcellPak UG C18色谱柱分离;检测波长:异绿原酸A、23-乙酰泽泻醇B和β-蜕皮甾酮检测波长分别设定348 nm、250 nm和208 nm;进样体积:10µL;柱温:28℃;以乙腈(A)-0.5%磷酸溶液(B)为洗脱溶剂,外标法定量检测。结果:异绿原酸A、β-蜕皮甾酮和23-乙酰泽泻醇B分别在浓度0.0767~7.67、0.0983~9.83和0.0197~1.97μg/mL范围内线性良好;回收率依次为100.05%、98.33%和99.42%;重复性和精密度RSD均在5.0%以内;供试品溶液在24h内稳定。结论:结果证明,此方法具有前处理简单等优点,可以用于眩晕宁颗粒制剂的质量控制。
Objective:A RP-HPLC method was established for the determination of 3,5-O-dicaffeoylquinic acid in Xuanyunning granules.Method:Capcell Pak UG C18 chromatographic column for separation;Detection wavelength:The detection wavelength of 3,5-O-dicaffeyl quinic acid,23-acetyl xerol B andβ-ecdysteroid is set at 348 nm,250 nm and 208 nm respectively;Column temperature:28℃;Injection volume:10μL;Acetonitrile is used as mobile phase a,0.5%phosphoric acid solutionis used as mobile phase B,external standard method for quantitative detection.Result:3,5-O-dicaffeyl quinic acid,23-acetyl xerol B andβ-ecdysteroid showed good linear relationships in the concentration range 0.0767~7.67、0.0983~9.83 and 0.0197~1.97μg/mL.Average recoveries were 100.05%,98.33%and 99.42%respectively;RSD(n=6)of precision and repeatability are within 5.0%,The test solution is stable within 24h.Conclusion:The results showed that this method has the advantages of simple pretreatment and can be used for the quality control of Xuanyunning granules.
作者
倪恒雨
Ni Hengyu(Huainan Food and Drug Inspection Center,232007)
出处
《现代科学仪器》
2023年第3期32-36,共5页
Modern Scientific Instruments
