UPLC法同时测定利肝隆颗粒中7种成分

Simultaneous determination of seven constituents in Liganlong Granules by UPLC

目的建立UPLC法同时测定利肝隆颗粒中新绿原酸、绿原酸、紫丁香苷、五味子醇甲、甘草酸铵、五味子甲素、五味子乙素的含量。方法该药物70%甲醇提取液的分析采用Waters ACQUITY UPLC C 18色谱柱(2.1 mm×100 mm,1.7μm);流动相甲醇-0.1%磷酸,梯度洗脱;体积流量0.3 mL/min;柱温35℃;检测波长254 nm。结果7种成分在各自范围内线性关系良好(r≥0.9997),平均加样回收率97.97%~99.92%,RSD 0.51%~1.60%。结论该方法快速、准确、灵敏,可用于利肝隆颗粒的质量控制。

AIM To establish a UPLC method for the simultaneous content determination of neochlorogenic acid,chlorogenic acid,syringoside,schisandrin,ammonium glycyrrhizinate,deoxyschizandrin andγ-schisandrin in Liganlong Granules.METHODS The analysis of 70%methanol extract of this drug was performed on a 35℃thermostatic Waters ACQUITY UPLC C 18 column(2.1 mm×100 mm,1.7μm),with the mobile phase comprising of methanol-0.1%phosphoric acid flowing at 0.3 mL/min in a gradient elution manner,and the detection wavelength was set at 254 nm.RESULTS Seven constituents showed good linear relationships within their own ranges(r≥0.9997),whose average recoveries were 97.97%-99.92%with the RSDs of 0.51%-1.60%.CONCLUSION This rapid,accurate and sensitive method can be used for the quality control of Liganlong Granules.