桑枝化学成分鉴定及其总黄酮提取工艺优化

Identification of chemical constituents and optimization of total flavonoids extraction process for Mori Ramulus

目的鉴定桑枝化学成分,并优化其总黄酮提取工艺。方法采用超高效液相色谱-四极杆-飞行时间高分辨率质谱(UPLC-Q-TOF-HRMS),在正负离子模式下采集数据,结合在线数据库和SCIEX工作站对桑枝醇提取物进行定性分析。在单因素试验基础上,采用响应面法优化提取工艺。结果共鉴定出57种成分。最佳条件为乙醇体积分数75%,料液比1∶21,提取时间70 min,提取温度75℃。结论UPLC-Q-TOF-HRMS、响应面法稳定可行,可用于桑枝定性分析及工艺优化。

AIM To identify the chemical constituents in Mori Ramulus and to optimize the extraction process for its total flavonoids.METHODS Using ultra high performance liquid chromatogrraphy-quadrupole time-of-flight high resolution mass spectrometry(UPLC-Q-TOF-HRMS)to collect the data in positive and negative ion mode for the qualitative analysis of the alcohol extract of Mori Ramulus,in combination with the online database and SCIEX workstation.On the basis of single factor test,the extraction process was optimized by response surface method.RESULTS Totally,fifty-seven constituents were identified.The optimal conditions were determined to be 75%for ethanol concentration,1∶21 for solid-liquid ratio,70 min for extraction time,and 75℃for extraction temperature.CONCLUSION The stable and feasible UPLC-Q-TOF-HRMS and response surface method can be used for the qualitative analysis and process optimization of Mori Ramulus.