UHPLC-ESI-Orbitrap-MS法分析龙脷叶化学成分

Identification on chemical constituents in Sauropus spatulifolius by UHPLC-ESI-Orbitrap-MS

目的建立超高效液相色谱串联质谱(UHPLC-ESI-Orbitrap-MS)法分析龙脷叶Sauropus spatulifolius Beille化学成分。方法该植物甲醇提取物的分析采用YMC-Pack ODS-AQ色谱柱(150 mm×2.0 mm,3μm);流动相0.1%甲酸-乙腈,梯度洗脱;体积流量0.2 mL/min;柱温35℃;电喷雾负离子模式。根据各成分高分辨数据结合对照品对所得成分进行结构鉴定,并对11批样品进行小分子化合物分布表征。结果从中鉴定了35个化合物,包括有机酸15个,苷类11个,酯类7个,其他2个,其特征性成分为咖啡酰葡糖酸、12-氧代亚油酸、14,16-二羟基-12-氧代油酸、阿魏酰葡糖酸。结论该方法合理可行,可为龙脷叶质量评价提供依据。

AIM To establish a ultra high performance liquid chromatography-electron spray ionization-orbitrap-mass spectrometer(UHPLC-ESI-Orbitrap-MS)method for the identification of the chemical constituents in Sauropus spatulifolius Beille.METHODS The analysis of methanol extract of this drug was performed on 35℃thermostatic YMC-Pack ODS-AQ columm(150 mm×2.0 mm,3μm),with the mobile phase comprising of 0.1%formic acid-acetonitrile flowing at 0.2 mL/min in a gradient elution manner;using an electrospray(ES1)ion source in negative ion mode.According to the high-resolution data of each component and the reference substance,the identifications of the structures of the obtained components,and the distribution features of small molecule compounds of eleven batches of samples were achieved.RESULTS Among the identified thirty-five compounds,including fifteen organic acids,eleven glycosides,seven esters and two others;the characteristic components were caffeioyl gluconic acid,12-oxo-linoleic acid,14,16-dihydroxy-12-oxo-oleic acid and ferulic gluconic acid.CONCLUSION This reasonable and feasible method provides a basis for the quality evaluation of S.spatulifolius.