基于正交试验法的3种大麻酚醇提工艺优化研究

Research of the Optimization of Ethanol Extraction Technology of Three Cannabinoids Based on Orthogonal Test Design

基于正交试验法,优化了3种大麻酚醇提工艺,通过提取时间、液料比值、提取温度与提取次数等单因素分析,建立了三因素三水平正交试验方案,确立了3种大麻酚的最佳醇提工艺:超声波功率300 W、提取时间7 min、液料比值25 mL/g、提取温度50℃、提取次数1次。3种大麻酚CBD、CBN、Δ9-THC提取率分别为3418μg/g、1716μg/g、5311μg/g,总提取率10.445 mg/g。此工艺可应用于大麻酚类成分HPLC定量检测样品的预处理中。

Based on orthogonal test design,the study optimizes ethanol extraction technology of three cannabinoids;does single factor analysis of extracting time,the liquid-solid ratio,extracting temperature and extracting times;establishes three factors and three levels orthogonal test scheme;and determines the best ethanol extraction technology of three cannabinoids:ultrasonic power 300 W,extraction time 7 min,liquid material ratio 25 mL/g,extraction temperature 50℃,and 1 time extraction.The extraction ratio of CBD,CBN andΔ9-THC of three cannabinoids are 3418μg/g,1716μg/g and 5311μg/g.The total extraction ratio is 10.445 mg/g.This technology can be applied to the pretreatment method of quantitative determination of cannabinoids.