金银花黄酮苷类化学成分研究

Flavonoid glycosides of Lonicera japonica flower buds

目的研究金银花(忍冬Lonicerajaponica的干燥花蕾)的黄酮苷类成分。方法采用溶剂提取法,结合硅胶、ODS、Sephadex LH-20等柱色谱和半制备高效液相法进行分离和纯化,并通过显色及NMR等波谱学方法进行鉴定。结果从金银花中分离到了9个黄酮苷类化合物,分别鉴定为3,5-二羟基-3′-甲氧基黄酮-7-O-β-D-葡萄糖苷(1)、槲皮素-3-O-葡萄糖苷(2)、3,5,3′-三羟基黄酮-7-O-β-D-葡萄糖苷(3)、棉花苷(4)、烟花苷(5)、水仙苷(6)、芦丁(7)、槲皮素-5-O-葡萄糖苷(8)、木犀草素-7-O-新橙皮糖苷(9)。结论化合物1和3为新化合物,分别命名为金银花黄酮F和金银花黄酮E;化合物2、4~6、8、9为首次从该属植物中分离得到。化合物1对人肝癌HuH7细胞的生长具有显著的抑制作用,其半数抑制浓度(median inhibitory concentration,IC_(50))为(43.4±1.1)μmol/L。

Objective To study flavonoid glycosides from the flower buds of Rendong(Lonicera japonica Thunb.)(Lonicera,Caprifoliaceae).Methods All flavonoid glycosides were systematically isolated and purified by solvent extraction,silica gel,ODS,Sephadex LH-20 column chromatography and semi-preparative high-performance liquid chromatography(HPLC),and identified by color reaction and NMR spectroscopy.Results A total of nine flavonoid glycosides were obtained from L.japonica flower buds,and identified as 3,5-dihydroxy-3′-methoxyflavone-7-O-β-D-glucopyranoside(1),quercetin-3-O-glucopyranoside(2),3,5,3′-trihydroxyflavone-7-O-β-D-glucopyranoside(3),quercimeritrin(4),nicotinflorin(5),narcissin(6),rutin(7),quercetin-5-O-glucoside(8),and luteolin-7-O-neohesperidoside(9).Conclusion Compounds 1 and 3 are new compounds.Compounds 2,4—6,8—9 were isolated in the genus Lonicera for the first time.Compound 1 showed inhibitory effect on HuH7 cells with IC_(50) of(43.4±1.1)μmol/L.