双酶水解对黑果腺肋花楸出汁率和花色苷的影响

Effects of Double Enzymatic Hydrolysis on Juice Yield and Anthocyanins of Aronia melanocarpa Elliot

为进一步提高黑果腺肋花楸果实出汁率和花色苷含量,采用双酶水解的方法对黑果腺肋花楸进行酶解,在考察温度、时间和添加量等单因素基础上,采用正交试验对黑果腺肋花楸的酶解参数进行优化。结果表明,在果胶酶与纤维素酶为1∶1的条件下,最优酶解工艺为酶添加量0.4%,酶解温度45℃,酶解时间3 h。此时,黑果腺肋花楸的出汁率为93.15%,花色苷含量为12.94 mg·g^(-1)。

In order to further improve the juice yield and anthocyanin content of Aronia melanocarpa Elliot fruit,the double enzymatic hydrolysis method was used.Based on single factor experiments such as enzymolysis temperature,enzymolysis time,and enzyme addition,the optimal enzymolysis parameters were optimized by orthogonal experiment.The results showed that the ratio of pectinase and cellulase was 1∶1,the optimal enzymolysis process was as follows:the enzyme addition was 0.4%,the enzymolysis temperature was 45℃,and the enzymolysis time was 3 h.The juice yield and anthocyanin content of Aronia melanocarpa Elliot were 93.15%and 12.94 mg·g^(-1) respectively.