复合微生物腐解菌剂的制备及其菌渣堆肥性能

Preparation of microbial decomposition compound bacterial agent and iTScomposting performance of spent mushroom substrate

为了更好地腐熟香菇菌渣,提高堆肥中有效磷和腐殖质含量,从废弃香菇菌渣中筛选出高效纤维素降解菌株YD4,使用纤维素降解菌里氏木霉DGW1及根际促生菌枯草芽孢杆菌SL-44和假单胞菌Rs-198,复配SL-44+Rs-198+YD4、SL-44+Rs-198+DGW1、Rs-198+DGW1+YD4、SL-44+DGW1+YD4、SL-44+Rs-198+YD4+DGW1等5种微生物菌剂组合。以2%的体积分数将不同组合菌剂分别接种并进行香菇菌渣堆肥研究。结果表明:与对照组相比,堆肥30 d后接种复合菌剂SL-44+Rs-198+YD4+DGW1的堆肥处理进入腐熟期最快为13 d,堆体电导率最低为1.37 mS·cm^(-1),腐植酸和富里酸的光密度之比(E4/E6)最低为6.11,有效磷含量和最大有机质降解率较高。接种菌剂SL-44+Rs-198+YD4+DGW1更好地促进了腐殖质的形成,提高了堆肥腐熟程度及稳定性。

In order to better rot mushroom residue and improve the content of available phosphorus and humus in compost,a high cellulose degrading strain YD4 was selected from the waste mushroom residue.The cellulose degrading bacterium Trichoderma reevi DGW1,rhizosphere promoting bacterium Bacillus subtilis SL-44 and Pseudomonas Rs-198 were used to compound five kinds of microbial agent combination:SL-44+Rs-198+YD4,SL-44+Rs-198+DGW1、Rs-198+DGW1+YD4、SL-44+DGW1+YD4、SL-44+Rs-198+YD4+DGW1.Different combinations of bacteria were inoculated with 2%volume fraction and the mushroom residue compost was carried out.The resulTSshow that:Compared with the control group,30 days after composting,the composting treatment inoculated SL-44+Rs-198+YD4+DGW1 entered the rot stage as soon as 13 days,the lowest electrical conductivity of the pile was 1.37 mS·cm^(-1),and the lowest optical density ratio(E4/E6)of humic acid to fulvic acid was 6.11.The content of available phosphorus and the maximum degradation rate of organic matter are higher.Inoculant SL-44+Rs-198+YD4+DGW1 promoted the formation of humus and improved the maturity and stability of compost.