光照与核黄素浓度对核黄素光动力灭活病原体效果的研究

Effect of light and riboflavin concentration on photodynamicinactivation of pathogens by riboflavin

目的探究不同光照时间条件下核黄素灭活病原体效果的影响。方法采用含水疱性口炎病毒(VSV)血浆分4组,分别添加核黄素浓度致终浓度为50、100、150μmol/L,0μmol/L为对照组。观察其在10、15、20、25 min的生长滴度,不进行光照的为对照组。用Reed-Muench法计算灭活前后培养后生长滴度,通过灭活后log减少因子评价不同浓度核黄素对灭活效果的影响。结果在无光照的条件下添加核黄素无病原体灭活效果,随着光照时间从15 min增加到25 min,无核黄素组VSV灭活效果增加;当光照小于15 min时,50~150μmol/L核黄素VSV灭活效果差异无统计学意义;当光照时间为15~25 min时,添加核黄素后VSV灭活效果增强,但不同浓度核黄素组间差异无统计学意义。结论在25 min内核黄素病原体灭活效果随光照时间的增加灭活效果越好,与核黄素浓度影响较小。

Objective To investigate the effect of riboflavin on inactivation of pathogens under different light time conditions.Methods Plasma containing vesicular stomatitis virus(VSV)was divided into 4 groups,and the final concentrations of riboflavin were 50,100,150μmol/L and 0μmol/L was used as the control group.The growth titers at 10,15,20 and 25 min were observed.The control group was treated with no light.The growth titers of VSV culture before and after inactivation were calculated by Reed-Muench method,and the effect of different concentrations of riboflavin on the inactivation effect was evaluated by log reduction factor after inactivation.Results Under the condition of no light,the addition of riboflavin had no pathogen inactivation effect.With the increase of light time from 15 min to 25 min,the inactivation effect of VSV in the non-riboflavin group increased.When the light was less than 15 min,there was no significant difference in the inactivation effect of 50 to 150μmol/L riboflavin VSV.When the light duration was 15 to 25 min,the inactivation effect of VSV was enhanced after the addition of riboflavin,but there was no difference between different concentrations of riboflavin groups.Conclusion The inactivation effect of riboflavin pathogen within 25 min is better with the increase of light time,and has little influence on riboflavin concentration.