SPP1、PD-L1的表达与M2巨噬细胞在肝细胞癌中的关系及对预后的影响研究

The correlation of the expression of SPP1, PD-L1 and M2-polarizedmacrophages in hepatocellular carcinoma and their influence on prognosis

目的探讨分泌性磷蛋白1(SPP1)及程序性死亡因子配体-1(PD-L1)在肝细胞癌(HCC)患者中的表达及与M2巨噬细胞的相关性以及对预后的影响。方法采用免疫组化法检测81例肝癌患者中SPP1、PD-L1以及CD163(M2巨噬细胞常用标志物)的表达,并分析SPP1、PD-L1和CD163的表达与患者临床特征相关性及与患者预后的关系,同时分析了SPP1、PD-L1与肝癌组织中M2巨噬细胞的相关性。结果SPP1、PD-L1和CD163的表达在肝癌患者中升高,SPP1、PD-L1和CD163的表达均与患者BCLC分期相关(P<0.05),与年龄、性别均无关。同时,SPP1的表达还在肿瘤直径大、肝内多发肿瘤的HCC中患者中增高;PD-L1的表达在肝内多发肿瘤、有乙肝病毒(HBV)感染的HCC患者中增高(P<0.05)。SPP1、PD-L1和CD163与预后相关,高表达患者的3年生存率(OSR)和总生存期(OST)低于低表达患者,BCLC分期、SPP1和PD-L1为独立预后因素。SPP1、PD-L1表达与M2巨噬细胞呈正相关。结论SPP1、PD-L1和CD163可作为判断肝癌生物学行为的参考指标,SPP1和PD-L1阳性表达提示肝癌患者可能预后不良。

Objective To evaluate the expression of programmed death receptor ligand-1(SPP1),programmed cell death(PD-L1)in hepatocellular carcinoma(HCC),their correlation with M2-polarized macrophages,and their impact on prognosis.Methods Immunohistochemistry(IHC)was used to detect the expression of SPP1,PD-L1,and CD163(commonly used markers of M2-polarized macrophages)in 81 HCC patients.The correlation between the expression of SPP1,PD-L1,and CD163 and the clinical characteristics of patients and their prognosis were analyzed.The relationship between SPP1,PD-L1,and M2-polarized macrophages in HCC tissue was also analyzed.Results The expression of SSP1,PD-L1 and CD163 increased in HCC tissues compared with para-carcinoma tissues.The expression of SSP1,PD-L1 and CD163 were all related to the BCLC staging(P<0.05),but were not related to age and sex.The expression of SSP1 was elevated in HCC patients with large tumor diameter and multiple intrahepatic tumors,PD-L1 was elevated in HCC patients with multiple intrahepatic tumors and HBV infection.SSP1,PD-L1 and CD163 were related to the prognosis,and high expression patients had lower 3-year OSR and OST compared to low expression patients.Cox analysis showed the BCLC staging,SPP1 and PD-L1 were independent prognostic factors.The expression of SPP1 and PD-L1 were positively correlated with M2 macrophages in HCC.Conclusion SPP1,PD-L1 and CD163 can be used to evaluate the biological behaviors of HCC,and the positive expression of SPP1 and PD-L1 suggests that HCC patients may have a poor prognosis.