不同品种厚皮甜瓜采后糖含量及糖代谢酶活性比较

Comparison of postharvest sugar content and sugar metabolizing enzyme activities of different varieties of thick-skinned melons

【目的】研究新疆不同品种甜瓜糖组分及其相关代谢酶活性差异。【方法】以9种新疆特色厚皮甜瓜为试材,提取和测定甜瓜中可溶性糖和转化酶(Ivr)、蔗糖磷酸合成酶(SPS)和蔗糖合成酶(SS),分析糖组分和糖代谢相关酶相关性。【结果】不同品种甜瓜的果糖、葡萄糖和蔗糖含量存在差异,果糖、葡萄糖和蔗糖含量范围分别为2.17~3.21、1.09~2.56和0.77~5.76 g/100 g;其中果糖含量最高的是黄梦脆,葡萄糖含量最高的是俊秀,蔗糖含量最高的是早醉仙。甘甜蜜宝的蔗糖磷酸合酶和蔗糖合成酶活性最高,分别为7.5和8.7μmol/(h·g),9种成熟甜瓜中转化酶处在低活性状态。【结论】不同品种厚皮甜瓜的糖组分和糖代谢相关酶存在着不同相关性。

【Objective】To compare the differences in the sugar content and their associated metabolic enzyme activity among different varieties of thick-skinned melons in Xinjiang.【Methods】9 varieties of thick-skinned melons were tested.The soluble sugar,invertase(Ivr),sucrose phosphate synthase(SPS)and sucrose synthase(SS)were extracted and determined.Meanwhile,the correlation between sugar content and their associated metabolic enzyme activity were analyzed.【Results】Results showed that significant differences exist in the contents of fructose,glucose and sucrose among different varieties of melons.Fructose,glucose and sucrose content ranged from 2.17 to 3.21 g/100 g,1.09 to 2.56 g/100 g and 0.77 to 5.76 g/100 g,respectively.Among which,Huang Meng cui has the highest fructose content,Jun xiu has the highest glucose content,and Zao zui xian has the highest sucrose content.The activities of SPS and SS of"Gan tian mi bao"were the highest,which were 7.5μmol/(h·g)and 8.7μmol/(h·g),respectively.Ivr of all the 9 melons have low activity.【Conclusion】There were different correlations between sugar content and their associated metabolic enzyme activity among different varieties of melons.