奶山羊CAEV感染血清抗体的消长规律

Pilot study on antibodies dynamics in dairy goats infected with caprine arthritis encephalitis virus

【目的】研究山羊感染关节炎-脑炎病毒(Caprine arthritis encephalitis virus,CAEV)后血清总抗体的消长规律,为CAEV检疫、净化方案的制订提供参考数据。【方法】采用我国分离的CAEV山东株(试验组)、参考株ATCC VR-905(参考株对照组)和细胞培养基(阴性对照组)经颈静脉注射感染3~5月龄关中奶山羊9只(每组3只),选用OIE推荐的CAEV/MVV总抗体检测试剂盒(IDEXX)定期检测抗体。【结果】阴性对照组始终为阴性,试验组和参考株对照组在感染后第24 d出现抗体转阳个体,试验组在第42 d时全部转阳,参考株对照组在第71 d时全部转阳,且在后续监测期内抗体OD值均处较高水平并。【结论】山羊感染CAEV后能够产生较好的体液免疫应答,前2个月内抗体水平差异较大,抗体刺激起来后将持续处在较高水平。血清中CAEV总抗体水平可作为山羊CAE检疫的判断标准,但必须进行间隔期不少于60 d的2次以上的检疫。

【Objective】To reveal the dynamic of total antibodies against Caprine Arthritis Encephalitis Virus(CAEV)in dairy goats.The results provide a reference data for the establishment of the CAEV quarantine and eradication program in China.【Methods】In this study,the CAEV Shandong strains(the experiment group)and reference strain(the reference control group)and cell culture medium(the negative control group)were injected intravenously into 9 dairy goats(3 dairy goats each group),respectively,and antibodies against CAEV were detected by CAEV/MVV total antibodies test kit(IDEXX)recommended by OIE.【Results】The antibodies were always negative in the negative control group during the experiment periods.The dairy goats in the experiment group and reference control group were first seroconversed on the 24th day,and the experiment group were all seroconversed on the 42nd day after infection,while the dairy goats in the reference control group were all seroconversed on the 71st day.All the seroconversed dairy goats persisted at high OD value of antibodies during the follow-up periods.【Conclusion】The results indicated that the dairy goats were elicited a well humoral immune response after being infected with CAEV.The antibody levels vary greatly in the first two months,but could continue to be at a higher level.The results indicated that the total serum antibody level of CAEV can be used as a criterion in goat CAE quarantine,but it must be quarantined more than twice with an interval of not less than 60 days.