天蓝巨土蜂的线粒体基因组及其系统发育含义(膜翅目:土蜂科)

The mitochondrial genome of Megascolia azurea(Christ)(Hymenoptera:Scoliidae) with its phylogenetic implications

天蓝巨土蜂Megascolia azurea (Christ,1791)是中国土蜂科巨土蜂属MegascoliaBetrem,1964的唯一代表,是鞘翅目姬独角仙Xylotrupesgideon(Linnaeus,1767)和椰蛀犀金龟Oryctesrhinoceros(Linnaeus,1758)的幼虫寄生蜂。我们测定了天蓝巨土蜂的线粒体基因组,其长度为16,335 bp且拥有38个线粒体基因。与其他动物线粒体相比,该线粒体基因包含一个额外的trnM基因。基因重排涉及蛋白编码基因和tRNA基因的倒置。通过最大似然法和贝叶斯推理分析两种方法以菜蛾盘绒茧蜂为外群对天蓝巨土蜂和其他8种针尾部昆虫的线粒体蛋白编码基因进行了系统发育分析,所获得的系统树拓扑结构表明,土蜂科是(蜜蜂科+(青蜂科+(沟土蜂科+蚁蜂科)+(蚁科+胡蜂科)))的姐妹群,而蛛蜂科与以上构成姐妹群并位于系统发育树的基部。

We sequenced and annotated the mitochondrial genome of Megascolia azurea(Christ,1791),the only representative of the genus Megascolia Betrem,1964 in the family Scoliidae in China.It is a larval parasitoid of the rhinoceros beetles Xylotrupes gideon(Linnaeus,1767) and Oryctes rhinoceros(Linnaeus,1758)(Coleoptera).The mitogenome is 16,335 bp in length and possesses 38 mitochondrial genes.However,unlike most animal mitochondrial genomes,it contains one extra trnM gene.Gene rearrangement shows an inversion event involving protein-coding and tRNA genes.The protein-coding genes in this sequence and another 8 species from the Aculeata were used for phylogenetic analysis by maximum likelihood(ML)analysis and Bayesian inference(BI) analyses using Cotesia vestalis from the family Braconidae as the outgroup.The topology of the resulted phylogenetic tree demonstrated that M.azurea is sister to Megacampsomeris prismatic,a species also belonging to family Scoliidae.Scoliidae was recovered as a sister to the clade of(Apidae +(Chrysididae +(Tiphiidae + Mutillidae) +(Formicidae + Vespidae))),followed by Pompilidae which was located at a basal position.