电针结合天麻素对PD模型大鼠黑质TH、TNF-α表达的影响

Effects of electroacupuncture combined with gastrodin on the expressions of TH and TNF-αin substantia nigra of PD model rats

目的观察电针结合天麻素对帕金森病(PD)大鼠黑质酪氨酸羟化酶(TH)和肿瘤坏死因子-α(TNF-α)表达的影响,探讨电针结合天麻素对PD的保护机制。方法将50只SD大鼠随机分为正常组、模型组、电针组、天麻素组和电针结合天麻素组,每组10只。PD动物模型采用颈背部皮下注射鱼藤酮来造模,天麻素组给予天麻素腹腔注射;电针组给予高频电针治疗;电针结合天麻素组先天麻素腹腔注射,再电针治疗;三组均治疗21 d。采用免疫组织化学法和Western Blot检测黑质TH和TNF-α的表达。结果与正常组相比,模型组大鼠黑质TH表达减少(P<0.01),天麻素、电针和电针结合天麻素治疗后TH表达增加(P<0.01);与天麻素组和电针组相比,电针结合天麻素组TH表达增加(P<0.05)。与正常组相比,模型组大鼠黑质TNF-α表达增加(P<0.01),天麻素、电针和电针结合天麻素治疗后黑质TNF-α表达减少(P<0.01),与天麻素组和电针组相比,电针结合天麻素组TNF-α表达减少(P<0.05)。结论电针结合天麻素可上调PD大鼠黑质TH表达,下调TNF-α表达,且作用强于天麻素或电针。

Objective To observe the effects of electroacupuncture combined with gastrodin on the expressions of tyrosine hydroxylase(TH)and tumor necrosis factor-α(TNF-α)in substantia nigra of rats with Parkinson’s disease(PD),aiming to explore the protective mechanism of electroacupuncture combining with gastrodin on rats with PD.Methods 50 SD rats were randomly divided into a normal group,a model group,an electroacupuncture group,a gastrodin group and a electroacupuncture+gastrodin group,with 10 rats in each group.The PD model was established by subcutaneous injection of rotenone into the neck and back.The gastrodin group was given gastrodin by intraperitoneal injection.The electroacupuncture group was treated with high-frequency electroacupuncture.The electroacupuncture+gastrodin group was treated with injection of gastrodin intraperitoneal followed by electroacupuncture.All three groups were treated for 21 days.This study employed immunohistochemistry and Western Blot to detect the expressions of TH and TNF-αin substantia nigra.Results Compared with the normal group,the model group had decreased TH expression in substantia nigra(P<0.01);and TH expression increased after the treatment with gastrodin,electroacupuncture and electroacupuncture combined with gastrodin(P<0.01).Compared with those of the gastrodin group and the electroacupuncture group,the TH expression increased in the electroacupuncture+gastrodin group(P<0.05).Compared with the normal group,the model group had increased expression of TNF-αin substantia nigra(P<0.01),but decreased expression of TNF-αafter the treatment with gastrodin,electroacupuncture and electroacupuncture combined with gastrodin(P<0.01).Compared with the gastrodin group and the electroacupuncture group,the electroacupuncture+gastrodin group had decreased TNF-αexpression(P<0.05).Conclusion Electroacupuncture combined with gastrodin can up-regulate TH expression and down-regulate TNF-αexpression in substantia nigra of PD rats.Its effect is superior to treatment with single gastrodin or electroacupuncture.