盐补骨脂对人HK-2、人Hep-G2及猪LLC-PK1的细胞毒性比较分析

Comparative analysis on the cytotoxicity of salt Psoraleae Fructus on human HK-2,human Hep-G2 and porcine LLC-PK1

对比分析盐补骨脂不同种提取物对3种细胞的毒性,本研究使用HK-2细胞、Hep-G2细胞及LLC-PK1细胞作为评价模型,采用CCK-8方法检测盐补骨脂水、醇提取物及其主要成分对3种细胞活力的影响。结果表明:1)盐补骨脂乙醇提取物对HK-2、Hep-G2及LLC-PK1细胞毒性均强于其水提取物。2)补骨脂甲素、补骨脂乙素、补骨脂定、异补骨脂定、甲基补骨脂黄酮A对HK-2、Hep-G2细胞毒性较强,且补骨脂甲素对HK-2细胞和Hep-G2细胞毒性要强于其他主要成分,而补骨脂素、异补骨脂素、补骨脂宁对HK-2、Hep-G2细胞毒性相对较小。综上,盐补骨脂水提取物、乙醇提取物对HK-2细胞、Hep-G2细胞及LLC-PK1细胞均具有一定的细胞毒性,且盐补骨脂乙醇提取物毒性更大;补骨脂甲素、补骨脂乙素、甲基补骨脂黄酮A、补骨脂素、异补骨脂素、异补骨脂定、补骨脂定对HK-2细胞、Hep-G2细胞具有一定的细胞毒性,且补骨脂甲素的毒性要大于补骨脂其他主要成分。本研究可为后续开展补骨脂或补骨脂有效成分的相关研究提供科学依据,在发挥其药理作用的同时,减少其毒性。

To compare and analyze the toxicity of various extracts of salt Psoraleae Fructus on three kinds of cells,HK-2 cells,Hep-G2 cells and LLC-PK1 cells were used as evaluation models in this study,and CCK-8 method was used to detect the effects of water extract of salt Psoraleae Fructus,alcohol extract and their main components on the viability of three kinds of cells.The results showed that:1)The ethanol extracts of salt-processed Psoraleae Fructus were more toxic to HK-2,Hep-G2 and LLC-PK1 cells than its aqueous extract.2)At the same time point and concentration,bavachin,isobavachalcone,psoralidin,isopsoralidin and bavachinin A were more toxic to HK-2 and Hep-G2 cells,and bavachin was more toxic to HK-2 and Hep-G2 cells than the other major components.In contrast,psoralen,isopsoralen,corylin were relatively less toxic to HK-2 and Hep-G2 cells.In conclusion,the water and ethanolic extracts of salt Psoraleae Fructus had certain cytotoxicity to HK-2 cells,Hep-G2 cells and LLC-PK1 cells,and its ethanolic extract was more toxic;bavachin,isobavachalcone,bavachinin A,psoralen,isopsoralen,psoralidin,corylin were more toxic to HK-2 cells.Based on the results of this study,it can provide scientific basis for subsequent research on psoralea or its effective components,and reduce its toxic effects while exerting its pharmacological effects.