非黑色素瘤皮肤癌患者外周血及肿瘤组织趋化因子水平及其临床意义

Chemokine Levels in Peripheral Blood and Cancer Tissues of Patients with Non-melanoma Skin Cancer and Their Clinical Significance

目的研究非黑色素瘤皮肤癌(NMSC)患者外周血及肿瘤组织中CC趋化因子配体5(CCL5)、CXC趋化因子配体13(CXCL13)、XC趋化因子配体1(XCL1)及CX3C趋化因子配体1(CX3CL1)的表达水平,并分析其临床意义。方法选取2017年1月—2019年12月本院收治的32例NMSC患者作为研究组,另选择同期本院收治的21例良性皮肤肿瘤患者作为对照组。对比2组外周血及肿瘤组织中CCL5、CXCL13、XCL1及CX3CL1表达水平的差异,并分析其与NMSC发生及肿瘤类型、分期、转移的相关性。结果研究组患者外周血CCL5、CXCL13、XCL1及CX3CL1水平明显高于对照组,差异有统计学意义(P<0.05);研究组患者肿瘤组织CCL5、CXCL13、XCL1及CX3CL1阳性表达率明显高于对照组,差异均有统计学意义(均P<0.05)。T_(3)期患者血清CCL5与XCL1表达水平明显高于T_(2)期与T_(1)期患者,T_(2)期患者CXCL13表达水平显著高于T_(1)期患者,发生转移者血清CCL5与XCL1表达水平明显高于未发生转移者,差异均有统计学意义(均P<0.05);NMSC患者肿瘤组织中CCL5与CX3CL1的阳性表达率与NMSC临床分期、转移相关,T_(3)期患者肿瘤组织CCL5与CX3CL1阳性表达率明显高于T_(1)期患者,发生转移者肿瘤组织CCL5与CX3CL1阳性表达率明显高于未发生转移者,差异均有统计学意义(均P<0.05)。结论趋化因子CCL5与CX3CL1表达的升高与NMSC的发生、进展与转移有关,可能在促进NMSC的发生发展中起到关键作用,可作为NMSC临床诊断的重要指标。

Objective In order to study the expression level of CC chemokine ligand 5(CCL5),CXC chemokine ligand 13(CXCL13),XC chemokine ligand 1(XCL1)and CX3C chemokine motif ligand 1(CX3CL1)in the peripheral blood and cancer tissues of patients with non-melanoma skin cancer(NMSC)and analyze their clinical significance.Methods Thirtytwo patients with NMSC admitted to our hospital from January 2017 to December 2019 were selected as the study group.In addition,21 cases of benign skin tumor admitted to our hospital during the same period were selected as the control group.The expressions of CCL5,CXCL13,XCL1 and CX3CL1 in peripheral blood and cancer tissues of the two groups were compared,and their correlations with the occurrence of malignant NMSC and tumor type,stage and metastasis were analyzed.Results The levels of CCL5,CXCL13,XCL1 and CX3CL1 in the peripheral blood of the patients in the study group were significantly higher than those in the control group(P<0.05).The positive expression rates of tumor tissues CCL5,CXCL13,XCL1 and CX3CL1 in the study group were significantly higher than those in the control group,the differences were statistically significant(P<0.05).The expression levels of serum CCL5,CXCL13,and XCL1 in patients with stage T_(3) were greatly higher than those in patients with stage T_(2) and stage T_(1)(P<0.05).The expression level of CXCL13 in stage T_(2) patients was significantly higher than that in stage T_(1)(P<0.05).The positive expression rates of CCL5 and CX3CL1 in cancer tissues of NMSC patients were related to the clinical stage and metastasis of NMSC.The positive expression rates of CCL5 and CX3CL1 in T_(3) patients cancer tissues were significantly higher than those in T_(1) patients,and the positive expression rates of CCL5 and CX3CL1 in tumor tissues of patients with metastasis were significantly higher than those of patients without metastasis(all P<0.05).Conclusion The increased expression of chemokines CCL5 and CX3CL1 is related to the occurrence,progression and metastasis of NMSC.It may play a key role in promoting the occurrence and development of NMSC,and can be used as an important indicator for the clinical diagnosis of NMSC development.