一株海泥来源真菌Talaromyces flavus 210331次级代谢产物及其抗氧化活性

Secondary Metabolites and Their Antioxidant Activities of a Sea Mud-derived Fungus Talaromyces flavus 210331

为从Talaromyces属中获得更多生物活性显著的次级代谢产物,对一株红树植物白骨壤(Avicennia marina)根际海泥来源真菌Talaromyces flavus 210331的次级代谢产物及其抗氧化活性进行研究。综合利用正相硅胶柱层析、Sephadex LH-20凝胶柱层析、半制备HPLC等色谱分离技术,对该菌发酵产物的乙酸乙酯萃取物进行次级代谢产物分离纯化;综合运用NMR、MS波谱对纯化的次级代谢产物进行结构鉴定,并评价鉴定次级代谢产物的抗氧化活性。结果显示从真菌T.flavus 210331中分离鉴定7个单体化合物,包括2个异香豆素类化合物hypoxymarin C(1)和peniciisocoumarin F(2),1个联烯类化合物penicilactone A(3),4个苯衍生物diorcinol(4)、p-hydroxybenzaldenhyde(5)、trans-ferulic acid(6)和agidol 10(7)。抗氧化活性测试结果表明化合物11~3具有显著的抗氧化活性,其IC_(50)值分别为(0.180±0.025)、(0.180±0.037)和(0.230±0.013)mmol/L,强于阳性对照trolox[IC50=(0.290±0.011)mmol/L]。

In order to obtain more secondary metabolites with significant biological activities from the genus Talaromyces,the secondary metabolites and biological activities of the fungus Talaromyces flavus 210331 isolated from sea mud of the rhizosphere of Avicennia marina were studied.The secondary metabolites were isolated and purified by normal phase sili‑ca gel column chromatography,Sephadex LH-20 gel column chromatography,semi-preparative HPLC and other chromatographic separation methods from the EtOAc extracts.Their structures were identified by comprehensive NMR,MS spectral and other spectral analysis methods.The antioxidant activities of the isolated secondary metabolites were also tested.Seven compounds were isolated and identified from T.flavus 210331,including two isocoumarins hypoxymarin C(1),peniciisocoumarin F(2)a diene penicilactone A(3),four benzene derivatives diorcinol(4),p-hydroxy-benzaldenhyde(5),trans-ferulic acid(6),and agidol 10(7).Compounds 1-3 showed good antioxidant activity,with the IC50 values of 0.18±0.025,0.18±0.037 and 0.23±0.013mmol/L,respectively,better than positive control trolox with the IC50 value of 0.29±0.011 mmol/L。