透骨血竭散对类风湿性关节炎模型大鼠炎症因子的影响

Effect of Tougu Xuejie powder on inflammatory factors in rats with rheumatoid arthritis

目的:探讨透骨血竭散内服对类风湿性关节炎(RA)大鼠膝关节滑膜组织炎症因子的影响。方法:选取60只雄性大鼠作为实验对象,按照体重区组化随机分组,每组10只。正常组、模型组每天按5.4 mg/kg给予0.9%氯化钠溶液灌胃;阳性对照组每日按5.4 mg/kg给予雷公藤多苷溶液灌胃;透骨血竭散高剂量组每天按1.6 g/kg给予透骨血竭散灌胃;透骨血竭散中剂量组每天按0.8 g/kg给予透骨血竭散灌胃;透骨血竭散低剂量组每天按0.4 g/kg给予透骨血竭散灌胃;各组均连续干预3周。观察六组大鼠膝关节滑膜组织病理变化;采用酶联免疫吸附实验法(ELISA)检测膝关节滑膜组织的TLR4、MyD88、NF-κB信号通路表达,根据表达情况分析其与RA的作用机制。结果:与正常组、阳性对照组和透骨血竭散各剂量组比较,造模第21、28、35天模型组大鼠足趾肿胀程度较高。干预3周后,阳性对照组、透骨血竭散高剂量组大鼠足趾肿胀程度降低,且低于模型组,差异有统计学意义(均P<0.05)。干预3周后,透骨血竭散高剂量组大鼠的关节炎症评分、足趾肿胀程度均降低,且均低于阳性对照组、透骨血竭散中剂量组、透骨血竭散低剂量组,差异有统计学意义(均P<0.05)。模型组关节滑膜组织TLR4 mRNA、MyD88 mRNA和NF-κBp65、NF-κB抑制因子α(I_(κB)α)、I_(κB)激酶复合物β(I_(κB)β)表达量高于正常组,差异有统计学意义(均P<0.05)。透骨血竭散各剂量组TLR4 mRNA、MyD88 mRNA和NF-κBp65、I_(κB)α、I_(κB)β表达量均低于阳性对照组(均P<0.05)。结论:透骨血竭散对RA的治疗作用与TLR4、MyD88和NF-κB信号通路密切相关。

Objective:To investigate effect of Tougu Xuejie powder on inflammatory factors of knee synovium in rats with rheumatoid arthritis(RA).Methods:Sixty male rats were randomly divided into 10 rats per group according to body mass area.The normal group and the model group were given 0.9%sodium chloride solution at 5.4 mg/kg daily.The positive control group were given tripterygium glycosides solution by gavage at rate of 5.4 mg/kg daily.The high dose group were given Tougu Xuejie powder at 1.6 g/kg daily by gavage.The medium dose group was given Tougu Xuejie powder at 0.8 g/kg daily by gavage.The low dose group was given Tougu Xuejie powder at 0.4 g/kg daily by gavage.All groups were subjected to continuous intervention for 3 weeks.The pathological changes of synovial tissue of knee joint in six groups were observed.The expressions of TLR4,MyD88 and NF-κB signaling pathways in synovial tissue of knee joint were detected by ELISA,and the mechanism of their interaction with RA was analyzed according to the expressions.Results:Compared with the normal group,the positive control group and each dose of Tougu Xuejie powder group,the toe swelling degree of rats in the model group at the 21st,28th and 35th day after modeling were higher.After 3 weeks of intervention,the degree of toe swelling of rats in the positive control group and the high dose of Tougu Xuejie powder group was lower than that in the model group,difference statistically significant(all P<0.05).After 3 weeks of intervention,the score of joint inflammation and the degree of toe swelling of rats in the high dose of Tougu Xuejie powder group were decreased,and they were all lower than those in the positive control group,the medium dose of Tougu Xuejie powder group and the low dose of Tougu Xuejie powder group,difference statistically significant(all P<0.05).The expressions of TLR4 mRNA,MyD88 mRNA,NF-κBp65,Iκbαand I_(κB)βin synovial tissue of the model group were higher than those in the normal group,difference statistically significant(all P<0.05).The expressions of TLR4 mRNA,MyD88 mRNA,NF-κBp65,I_(κB)αand I_(κB)βin each dose of Tougu Xuejie powder group were lower than those in the positive control group,difference statistically significant(all P<0.05).Conclusion:The therapeutic effect of Tougu Xuejie powder on RA is closely related to TLR4,MyD88 and NF-κB signaling pathway.