结直肠腺癌中超保守RNA-134的表达及其临床病理意义

Expression of Ultra Conservative RNA-134 in Colorectal Cancer and Its Clinicopathological Significance

目的:研究结直肠腺癌(CRAC)中超保守RNA-134(uc.134)的表达及其临床病理意义,探索uc.134对结直肠癌细胞转移的影响。方法:收集扬州大学附属医院手术切除的结直肠癌81例标本及临床特征。RNA原位分子杂交技术(RISH)检测uc.134在CRAC组织中的阳性表达,统计分析uc.134的阳性表达与CRAC病理特征的关联性。采用Transwell实验检测uc.134对大肠癌细胞侵袭迁移的影响。结果:RISH实验结果表明CRAC组织中uc.134主要为阳性表达(62/81),而癌旁正常大肠组织主要为阴性表达(7/81),两组间阳性比例有明显差异(P<0.05)。统计分析得出uc.134的阳性表达与CRAC的病理分级、浸润深度和淋巴结转移密切相关(P均<0.05),而与患者年龄、性别、肿瘤大小、发病部位和TNM分期之间无关(均P>0.05)。Transwell实验提示过表达uc.134促进CRAC细胞的迁移,而降低uc.134表达则抑制了癌细胞的迁移(P均<0.05)。结论:在CRAC中uc.134表达水平上调,是一个新的致癌基因,同时也是一个促进癌细胞侵袭、迁移和淋巴结转移的分子;uc.134有可能成为CRAC早期诊断的分子标志物及治疗转移的靶点。

Objective:To study the expression of super conservative RNA-134(uc.134)in colorectal adenocarcinoma(CRAC)and its clinicopathological significance.Methods:The clinical features of 81 cases of colorectal cancer resected surgically in the Affiliated Hospital of Yangzhou University were collected.The positive expression of uc.134 in CRAC tissues was detected by RNA in situ hybridization(RISH),and the correlation between the positive expression of uc.134 and the pathological characteristics of CRAC was statistically analyzed.Transwell experiment was used to detect uc.134 on invasion and migration of colorectal cancer cells.Results:RISH results showed that uc.134 in CRAC tissue was mainly positive(62/81),while the normal intestinal tissues adjacent to cancer were mainly negative(7/81).There was a significant difference between the two groups(P<0.05).Statistical analysis shows that the positive expression of uc.134 was closely related to the pathological grade,depth of invasion and lymph node metastasis of CRAC(all P<0.05).And there was no correlation between age,sex,tumor size,disease site and TNM stage(all P>0.05).Transwell experiment showed that overexpression of uc.134 promoted the migration of CRC cells,while decreasing the expression of uc.134 inhibited the migration of cancer cells(all P<0.05).Conclusion:In CRAC,the up-regulated expression of uc.134 is a new oncogene and a molecule that can promote the invasion,migration and lymph node metastasis of cancer cells;it may become a molecular marker for early diagnosis of CRAC and a target for therapeutic metastasis.