“病-证-治”结合自然流产模型研究进展及在单细胞多模态组学的应用趋势

Advances in“Disease-syndrome-therapy”Abortion Models and Trendsin the Application of Single-cell Multimodal Omics

自然流产发病率逐年递增,其病因繁杂,治疗手段有限,对社会稳定性危害严重。“病-证-治”结合研究模式能显著指导中医安胎临床疗效的提高,但一直缺乏关键且公认的诊治评价标记物,待进一步探索建立科学统一的评价标准体系。拟通过收集现有“病-证-治”结合自然流产动物模型,移植完善模型赋值评价指标,评判自然流产动物模型与中、西医临床病证特点的吻合程度,比较不同自然流产动物模型之构建方式、选择对象及评价指标等的优劣;此外,探索中医药实验研究前沿趋势,针对自然流产中医动物模型分子生物学标志物研究现状及相关瓶颈,提出单细胞多模态组学研究思路突破自然流产“病-证-治”的相关评价缺陷,从单个细胞水平分析母胎界面组织中各种细胞类型、细胞亚群、时空轨迹及基因表达的差异性等因素。为病-证模型、藏象生物学、新型靶向药物的深入研究提供精准指导及模型动物平台支撑,对建立具有稳定性和重复性的病证结合自然流产动物模型与评价指标体系提供依据,有利于中医妇科生殖领域动物模型研究的长远发展。

The incidence of spontaneous abortion(SAB)has been increasing year by year,and its etiology is complex,with limited treatment options,posing a serious threat to social stability.The“disease-syndrome-therapy”combination research model can significantly improve the clinical efficacy of traditional Chinese medicine(TCM)for preventing miscarriage,but there has always been a lack of key and recognized diagnostic and treatment evaluation markers,which need to be further explored to establish a scientific and unified evaluation standard system.It is proposed to collect existing“disease-syndrome-therapy”combination SAB animal models,transplant and improve the model evaluation indicators,evaluate the degree of match between SAB animal models and the clinical characteristics of TCM and Western medicine disease-syndrome,and compare the advantages and disadvantages of different SAB animal model construction methods,target selection,and evaluation indicators.In addition,the frontiers of TCM experimental research will be explored,and a singlecell multi-modal omics research strategy will be proposed to break through the related evaluation defects of the SAB"prescriptioncontradiction"and analyze the differences in various cell types,cell subpopulations,spatiotemporal trajectories,and gene expression at the single-cell level in the mother-fetal interface tissue.This will provide accurate guidance and model animal platform support for the indepth study of disease-syndrome models,Zang-fu biology,and novel targeted drugs.It will also provide a basis for establishing a stable and repeatable“disease-syndrome-therapy”combination SAB animal model and evaluation indicator system,which is beneficial for the long-term development of TCM reproductive animal model research.