UPLC法同时测定心源素胶囊中6种有效成分的含量

Simultaneous Determination of Six Active Ingredients in XinYuanSu Capsules by UPLC

目的建立UPLC法同时测定心源素胶囊中6种有效成分的含量。方法采用Waters Acquity UPLC BEH C 18(2.1×50 mm,1.7μm)色谱柱;以乙腈-水为流动相,梯度洗脱,流速为0.3 mL·min^(-1),柱温为40℃;203 nm和250 nm双波长检测,进样量为2μL。结果三七皂苷R_(1)、人参皂苷Rg_(1)、人参皂苷Re、人参皂苷Rb_(1)、五味子醇甲和五味子乙素分别在质量浓度0.0239~2.3866 mg·mL^(-1)、0.0247~2.4722 mg·mL^(-1)、0.0284~2.8378 mg·mL^(-1)、0.0248~2.4744 mg·mL^(-1)、0.0048~0.4780 mg·mL^(-1)和0.0056~0.5560 mg·mL^(-1)范围内与峰面积线性关系良好,相关系数均为1;平均回收率分别为99.9%、100.3%、99.4%、99.2%、98.0%和95.7%,RSD分别为2.6%、1.5%、1.1%、1.5%、2.0%和1.4%(n=6)。结论所建立的方法简便、快捷,结果准确,可用于心源素胶囊的质量控制。

OBJECTIVE To establish a UPLC method for simultaneous determination of six active ingredients in XinYuanSu capsules.METHODS A Waters Acquity UPLC BEH C 18(2.1×50 mm,1.7μm)column was adopted.Acetonitrile-water was used as mobile phase by gradient elution and the flow rate was 0.3 mL·min^(-1)with column temperature of 40℃.The detection wavelength was 203 nm and 250 nm and the injection volume was 2μL.RESULTS The linear ranges of notoginsenoside R_(1),ginsenoside Rg_(1),ginsenoside Re,ginsenoside Rb_(1),schisandrol A and schisandrin B were 0.0239-2.3866 mg·mL^(-1),0.0247-2.4722 mg·mL^(-1),0.0284-2.8378 mg·mL^(-1),0.0248-2.4744 mg·mL^(-1),0.0048-0.4780 mg·mL^(-1)and 0.0056-0.5560 mg·mL^(-1),respectively,with correlation coefficients of 1.The average recoveries were 99.9%,100.3%,99.4%,99.2%,98.0%and 95.7%with RSDs of 2.6%,1.5%,1.1%,1.5%,2.0%and 1.4%,respectively(n=6).CONCLUSION The established method is simple,rapid and accurate,which can be used for the quality control of Xinyuansu capsules.