痛风金黄散的薄层鉴别及含量测定研究

Study on TLC Identification and Content Determination of Tongfeng Jinhuang Powder

目的:建立痛风金黄散的薄层鉴别及含量测定。方法:采用薄层色谱法对黄柏、大黄、薄荷三味药材进行定性鉴别;采用HPLC法测定黄柏有效成分盐酸小檗碱含量,并进行方法学考察。结果:三味中药的薄层色谱分离度较好,斑点清晰,阴性对照无干扰。HPLC测定结果显示,盐酸小檗碱在3.0666~30.6661μg/ml浓度范围内线性关系良好,平均加样回收率为96.98%(RSD=2.46%,n=6)。结论:所建立的方法简单,灵敏,结果准确可靠,可以有效的控制痛风金黄散的质量。

Objective:To establish themethod of TLC identification and content determination for Tongfeng Jinhuang powder.Methods:The three medicinal materials of Hellodendri Chinensis Cortex,Rhei Radix Et Rhizoma and Menthae Haplocalycis Herba were qualitatively identified by TLC.Meanwhile,the content of berberine hydrochloride and the active ingredient of phellodendri were determined by HPLC,and the methodology was investigated.Results:The TLC identification spots of the three traditional Chinese medicines were clear,the separation was good,and the negative control had no interference.The results of HPLC assay showed that berberine hydrochloride had a good linear relationship in the concentration range of 3.0666~30.6661μg/mL.The average recovery of berberine hydrochloride was 96.98%(RSD=2.46%,n=6).Conclusion:The method is simple,sensitive,accurate and reliable,and can effectively control the quality of Tongfeng Jinhuang powder.